Copper (II) and 2,2 '-Bipyridine Complexation Improves Chemopreventive Effects of Naringenin against Breast Tumor Cells

Autor(es)

Conceicao Filho, Julio Cesar ; Franceschini Sarria, Andre Lucio ; Becceneri, Amanda Blanque ; Fuzer, Angelina Maria ; Batalhao, Jaqueline Raquel ; Paranhos da Silva, Caio Marcio ; Carlos, Rose Maria ; Vieira, Paulo Cezar ; Fernandes, Joao Batista ; Cominetti, Marcia Regina

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 9, p. 13-pg., 2014-09-05.

Resumo

Cancer is the second leading cause of death worldwide and there is epidemiological evidence that demonstrates this tendency is emerging. Naringenin (NGEN) is a trihydroxyflavanone that shows various biological effects such as antioxidant, anticancer, anti-inflammatory, and antiviral activities. It belongs to flavanone class, which represents flavonoids with a C6-C3-C6 skeleton. Flavonoids do not exhibit sufficient activity to be used for chemotherapy, however they can be chemically modified by complexation with metals such as copper (Cu) (II) for instance, in order to be applied for adjuvant therapy. This study investigated the effects of Cu(II) and 2,29-bipyridine complexation with naringenin on MDA-MB-231 cells. We demonstrated that naringenin complexed with Cu(II) and 2,29-bipyridine (NGENCuB) was more efficient inhibiting colony formation, proliferation and migration of MDA-MB-231 tumor cells, than naringenin (NGEN) itself. Furthermore, we verified that NGENCuB was more effective than NGEN inhibiting pro-MMP9 activity by zymography assays. Finally, through flow cytometry, we showed that NGENCuB is more efficient than NGEN inducing apoptosis in MDA-MB-231 cells. These results were confirmed by gene expression analysis in real time PCR. We observed that NGENCuB upregulated the expression of pro-apoptotic gene caspase-9, but did not change the expression of caspase-8 or anti-apoptotic gene Bcl-2. There are only few works investigating the effects of Cu(II) complexation with naringenin on tumor cells. To the best of our knowledge, this is the first work describing the effects of Cu(II) complexation of a flavonoid on MDA-MB-231 breast tumor cells. (AU)

• Processo FAPESP: 08/56758-0 - Triagem em larga escala de produtos de origem natural com atividade antitumoral em cultura de células de mamífero
• Beneficiário: Márcia Regina Cominetti
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores