Lack of beta(2)-adrenoceptors aggravates heart failure-induced skeletal muscle myopathy in mice

Autor(es)

Voltarelli, Vanessa A. ; Bechara, Luiz R. G. ; Bacurau, Aline V. N. ; Mattos, Katt C. ; Dourado, Paulo M. M. ; Bueno, Carlos R., Jr. ; Casarini, Dulce E. ; Negrao, Carlos E. ; Brum, Patricia C.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE; v. 18, n. 6, p. 11-pg., 2014-06-01.

Resumo

Skeletal myopathy is a hallmark of heart failure (HF) and has been associated with a poor prognosis. HF and other chronic degenerative diseases share a common feature of a stressed system: sympathetic hyperactivity. Although beneficial acutely, chronic sympathetic hyperactivity is one of the main triggers of skeletal myopathy in HF. Considering that (2)-adrenoceptors mediate the activity of sympathetic nervous system in skeletal muscle, we presently evaluated the contribution of (2)-adrenoceptors for the morphofunctional alterations in skeletal muscle and also for exercise intolerance induced by HF. Male WT and (2)-adrenoceptor knockout mice on a FVB genetic background (2KO) were submitted to myocardial infarction (MI) or SHAM surgery. Ninety days after MI both WT and 2KO mice presented to cardiac dysfunction and remodelling accompanied by significantly increased norepinephrine and epinephrine plasma levels, exercise intolerance, changes towards more glycolytic fibres and vascular rarefaction in plantaris muscle. However, 2KO MI mice displayed more pronounced exercise intolerance and skeletal myopathy when compared to WT MI mice. Skeletal muscle atrophy of infarcted 2KO mice was paralleled by reduced levels of phosphorylated Akt at Ser 473 while increased levels of proteins related with the ubiquitin--proteasome system, and increased 26S proteasome activity. Taken together, our results suggest that lack of (2)-adrenoceptors worsen and/or anticipate the skeletal myopathy observed in HF. (AU)

• Processo FAPESP: 08/56483-1 - Papel dos receptores B2-adrenérgicos nas alterações músculo-esqueléticas desencadeadas pela insuficiência cardíaca
• Beneficiário: Vanessa Azevedo Voltarelli
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/50048-1 - Bases celulares e funcionais do exercício físico na doença cardiovascular
• Beneficiário: Carlos Eduardo Negrão
• Modalidade de apoio: Auxílio à Pesquisa - Temático