| Texto completo | |
| Autor(es): |
Santos, Clelton A.
;
Janissen, Richard
;
Toledo, Marcelo A. S.
;
Beloti, Lilian L.
;
Azzoni, Adriano R.
;
Cotta, Monica A.
;
Souza, Mete P.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1854, n. 10, p. 10-pg., 2015-10-01. |
| Resumo | |
The intriguing roles of the bacterial Tol-Pal trans-envelope protein complex range from maintenance of cell envelope integrity to potential participation in the process of cell division. In this study, we report the characterization of the XfFolB and XfPal proteins of the Tol-Pal complex of Xylella fastidiosa. X. fastidiosa is a major plant pathogen that forms biofilms inside xylem vessels, triggering the development of diseases in important cultivable plants around the word. Based on functional complementation experiments in Escherichia coli tolB and pal mutant strains, we confirmed the role of xftolB and xfpal in outer membrane integrity. In addition, we observed a dynamic and coordinated protein expression profile during the X. fastidiosa biofilm development process. Using small-angle X-ray scattering (SAXS), the low-resolution structure of the isolated XfToIB-XfPal complex in solution was solved for the first time. Finally, the localization of the XfFolB and XfPal polar ends was visualized via immunofluorescence labeling in vivo during bacterial cell growth. Our results highlight the major role of the components of the cell envelope, particularly the TolB-Pal complex, during the different phases of bacterial biofilm development (C) 2015 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 09/53021-0 - Biofilmes de Xylella fastidiosa: análise por espectroscopia de força e microscopia de fluorescência |
| Beneficiário: | Richard Janissen |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 08/55690-3 - Expressão, purificação e caracterização estrutural e funcional de proteínas relacionadas à patogenicidade de Xylella fastidiosa |
| Beneficiário: | Clelton Aparecido dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/51580-4 - Caracterização funcional e molecular de proteínas potencialmente relacionadas a fitopatogenicidade da bactéria formadora de biofilme Xylella fastidiosa |
| Beneficiário: | Anete Pereira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 01/07533-7 - Resolução da estrutura tridimensional de proteínas relacionadas à patogenicidade de Xyllela fastidiosa |
| Beneficiário: | Anete Pereira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |