Beneficial Effects of Physical Exercise on Functional Capacity and Skeletal Muscle Oxidative Stress in Rats with Aortic Stenosis-Induced Heart Failure

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Gomes, Mariana Janini ; Martinez, Paula Felippe ; Salome Campos, Dijon Henrique ; Pagan, Luana Urbano ; Bonomo, Camila ; Ruiz Lima, Aline Regina ; Damatto, Ricardo Luiz ; Cezar, Marcelo D. M. ; Damatto, Felipe Cezar ; Rosa, Camila Moreno ; Garcia, Camila Marchiolli ; Abreu Reyes, David Rafael ; Henrique Fernandes, Ana Angelica ; Fernandes, Denise Castro ; Laurindo, Francisco Rafael ; Okoshi, Katashi ; Okoshi, Marina Politi

• Tipo de documento: Artigo Científico
• Fonte: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2016, p. 12-pg., 2016-01-01.

Resumo

Objective. We evaluated the influence of exercise on functional capacity, cardiac remodeling, and skeletal muscle oxidative stress, MAPK, and NF-kappa B pathway in rats with aortic stenosis-(AS-) induced heart failure (HF). Methods and Results. Eighteen weeks after AS induction, rats were assigned into sedentary control (C-Sed), exercised control (C-Ex), sedentary AS (AS-Sed), and exercised AS (AS-Ex) groups. Exercise was performed on treadmill for eight weeks. Statistical analyses were performed with Goodman and ANOVA or Mann-Whitney. HF features frequency and mortality did not differ between AS groups. Exercise improved functional capacity, assessed by maximal exercise test on treadmill, without changing echocardiographic parameters. Soleus cross-sectional areas did not differ between groups. Lipid hydroperoxide concentration was higher in AS-Sed than C-Sed and AS-Ex. Activity of antioxidant enzymes superoxide dismutase and glutathione peroxidase was changed in AS-Sed and restored in AS-Ex. NADPH oxidase activity and gene expression of its subunits did not differ between AS groups. Total ROS generation was lower in AS-Ex than C-Ex. Exercise modulated MAPK in AS-Ex and did not change NF-kappa B pathway proteins. Conclusion. Exercise improves functional capacity in rats with AS-induced HF regardless of echocardiographic parameter changes. In soleus, exercise reduces oxidative stress, preserves antioxidant enzyme activity, and modulates MAPK expression. (AU)

• Processo FAPESP: 12/22485-3 - Influência do exercício físico sobre o estresse oxidativo, as MAPK e o NF-ºB no músculo sóleo de ratos com insuficiência cardíaca induzida por estenose aórtica
• Beneficiário: Mariana Janini Gomes
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/21687-1 - Influência da N-acetilcisteína sobre a sinalização de apoptose na musculatura esquelética de ratos com insuficiência cardíaca crônica
• Beneficiário: Marina Politi Okoshi
• Modalidade de apoio: Auxílio à Pesquisa - Regular