Increased concentration of 8-hydroxy-2 '-deoxyguanosine in follicular fluid of infertile women with endometriosis

Autor(es)

Da Broi, Michele G. ; de Albuquerque, Felipe O. ; de Andrade, Aline Z. ; Cardoso, Rafaela L. ; Jordao Junior, Alceu A. ; Navarro, Paula A.

• Tipo de documento: Artigo Científico
• Fonte: Cell and Tissue Research; v. 366, n. 1, p. 12-pg., 2016-10-01.

Resumo

Impaired oocyte quality and oxidative stress might be involved in the pathogenesis of endometriosis-related infertility. To improve our understanding of the role of oxidative stress in this condition, we compare eight oxidative stress markers from each stage, including the simultaneous analysis of lipids, proteins and DNA damage, in the serum and follicular fluid of infertile women with endometriosis and infertile controls undergoing controlled ovarian stimulation for intracytoplasmic sperm injection. In total, 87 serum samples (43 with endometriosis, 44 controls) and 61 follicular fluid samples (29 with endometriosis, 32 controls) free of blood contamination upon visual inspection and presenting granulosa cells alone or granulosa cells plus a retrieved mature oocyte were collected on the day of oocyte retrieval. Total hydroperoxides, malondialdehyde, advanced oxidation protein products, glutathione, superoxide dismutase (SOD) and total antioxidant capacity (TAC) were determined by spectrophotometry, vitamin E by high-performance liquid chromatography and 8-hydroxy-2'-deoxyguanosine (8OHdG) by enzyme-linked immunosorbent assay. The endometriosis group showed higher serum concentrations of glutathione and SOD, lower serum concentrations of TAC and higher follicular concentrations of 8OHdG and vitamin E compared with infertile controls. These data indicate both systemic and follicular oxidative stress in infertile patients with endometriosis. For the first time, we demonstrate the presence of oxidative DNA damage, represented by higher 8OHdG concentrations in the follicular microenvironment of these patients, possibly related to compromised oocyte quality and associated with the pathogenesis of endometriosis-related infertility. (AU)

• Processo FAPESP: 08/58197-6 - Balanço oxidante e antioxidante de mulheres inférteis com endometriose comparadas a controle (fatores masculino e tubário de infertilidade) submetidas a estimulação ovariana e influência nos resultados
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/56373-1 - Avaliação da peroxidação lipídica, dos níveis de vitamina E e da capacidade antioxidante total no soro de mulheres inférteis com endometriose e infertilidade por fatores tubário e masculino e associação com a resposta à estimulação e os resultados da injeção intracitoplasmática de espermatozoides
• Beneficiário: Rafaela Lopes Cardoso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/15866-6 - Annual Meeting of the American Society for Reproductive Medicine - ASRM2014
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior