| Texto completo | |
| Autor(es): |
de Oliveira, Gilberto Santos
;
Kawahara, Rebeca
;
Rosa-Fernandes, Livia
;
Avila, Carla C.
;
Larsen, Martin R.
;
Pereira Alves, Joao Marcelo
;
Palmisano, Giuseppe
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY; v. 418, p. 16-pg., 2017-07-01. |
| Resumo | |
Chagas disease, also known as American trypanosomiasis, is a neglected tropical disease caused by the Trypanosoma cruzi parasite. In order to develop diagnostic and therapeutic solutions, there has been an intense investigation on the parasite biology using omics technologies such as genomics, transcriptomics lipidomics and proteomics. In particular, large scale mass spectrometry-based proteomics studies have allowed the identification and quantification of proteins and selected PTMs in different biological conditions. In this study, we investigated the unassigned MS/MS spectra commonly observed in large scale bottom up proteomics experiments looking at the T. cruzi (Sylvio X10/1) proteome. A deep proteomics data analysis using proteogenomic and unrestrictive PTMs search approaches allowed us to annotate 30% more MS/MS spectra and identify novel DNA coding regions and uncharacterized PTMs in Trypanosomatids, such as protein arginylation. Overall, this study shows: (1) the importance of assigning protein modifications, analytical artefacts and PTMs, in large-scale mass spectrometry-based proteomics data to deeply profile the trypanosomatids proteome. (2) The need of a better characterization of the influence of sample preparation steps on the identification of proteins and protein modifications. (3)The identification of novel DNA coding regions in T. cruzi. (4) The discovery of protein arginylation in trypanosomatids. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 14/06863-3 - Modificações pós-traducionais para o diagnóstico de câncer e doenças parasitárias: abordagens metodológicas e implicações biológicas |
| Beneficiário: | Giuseppe Palmisano |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 15/02866-0 - Descoberta e verificação de glicopeptídeos intactos como candidatos à biomarcadores em câncer de próstata utilizando abordagens de proteômica-baseada em espectrometria de massas |
| Beneficiário: | Rebeca Kawahara Sakuma |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |