Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection

Autor(es)

Souza, Sivoney F. ; Kogikoski, Sergio, Jr. ; Silva, Emerson R. ; Alves, Wendel A.

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 28, n. 9, p. 11-pg., 2017-09-01.

Resumo

Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions. (AU)

• Processo FAPESP: 13/12674-6 - Investigações estruturais em sistemas-modelo à base de peptídeos anfifílicos
• Beneficiário: Emerson Rodrigo da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção
• Beneficiário: Wendel Andrade Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/01933-8 - Matrizes biomiméticas obtidas por eletrofiação: possível aplicação na detecção de NO
• Beneficiário: Sergio Kogikoski Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto