| Texto completo | |
| Autor(es): Mostrar menos - |
Palanco, Ana Cerrejn
;
Singulani, Junya de lacorte
;
Costa-Orlandi, Caroline Barcelos
;
Gullo, Fernanda Patricia
;
Strohmayer Lourencetti, Natalia Manuela
;
Gomes, Paulo Cesar
;
Ayusso, Gabriela Miranda
;
Dutra, Luiz Antonio
;
Bolzani, Vanderlan da Silva
;
Regasini, Luis Octavio
;
Soares Mendes-Giannini, Maria Jose
;
Fusco-Almeida, Ana Marisa
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FUTURE MICROBIOLOGY; v. 12, n. 13, p. 12-pg., 2017-10-01. |
| Resumo | |
Aim: This work aimed to evaluate the activity of 3'-hydroxychalcone against Cryptococcus gattii in planktonic and biofilm forms and their toxicity using alternative animal models. Materials & methods: Minimum inhibitory concentration and minimum fungicide concentration were determined. Biofilm formation and the susceptibility tests were performed by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-5-[carbonyl(phenylamino)]2H-tetrazolium hydroxide assay. Toxicity and efficacy were checked in Danio rerio and Galleria mellonella models. Results: The compound 3'-hydroxychalcone showed fungicidal activity against C. gattii in both planktonic and biofilm forms. The toxicity in zebrafish embryos revealed a low lethal concentration. In G. mellonella, the compound did not show antifungal activity and larvae toxicity. Conclusion: Because of the activity of 3'-hydroxychalcone against C. gattii in vitro, molecular modifications should be made to improve efficacy and to reduce toxicity in vivo. (AU) | |
| Processo FAPESP: | 14/10446-9 - Pesquisa de novas terapias e de biomarcadores na paracoccidioidomicose: estudo da atividade antifúngica de galatos de alquila em modelos alternativos e camundongos e identificação de microRNAs circulantes |
| Beneficiário: | Junya de Lacorte Singulani |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 14/18330-0 - Preparação e avaliação biológica de híbridos curcumina-cinamaldeído como inibidores da divisão celular bacteriana |
| Beneficiário: | Luis Octávio Regasini |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |