Development and Validation of a Rapid and Reliable RP-HPLC-PDA Method for the Quantification of Six Diterpenes in Copaifera duckei, Copaifera reticulata and Copaifera multijuga Oleoresins

Autor(es)

Carneiro, Luiza J. ; Bianchi, Thamires C. ; da Silva, Jonas J. M. ; Oliveira, Larissa C. ; Borges, Carly H. G. ; Lemes, Danieli C. ; Bastos, Jairo K. ; Veneziani, Rodrigo C. S. ; Ambrosio, Sergio R.

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 29, n. 4, p. 9-pg., 2018-04-01.

Resumo

Oleoresins from Copaifera species are extensively used in folk medicine in Brazil, which are employed mainly in the production of cosmetic formulations in Brazil, North America and Europe. Considering the lack of validated analytical methods for the analyses of diterpenes in Copaifera oleoresins, it was developed a validated and reliable reversed-phase high-performance liquid chromatography with photodiode array detection (RP-HPLC-PDA) method for the analysis of six diterpenes, including: (-)-polyalthic acid; (13E)-ent-labda-7,13-dien-15-oic acid; ent-8(17) -labden-15,18-dioic acid; (-)-copalic acid; (-)-3 beta-acetoxycopalic acid and (-)-3 beta-hydroxycopalic acid. These compounds were isolated from C. duckei, C. reticulata and C. multijuga oleoresins by chromatographic means. The analytical curves were linear with regression coefficients (r(2)) between 0.9903 and 0.9999. The limits of detection (LOD) and quantification (LOQ) values were 0.35 to 3.09 mu g mL(-1) and 1.05 to 9.36 mu g mL(-1), respectively. The method also displayed good precision and accuracy. The developed analytical method is reliable and a useful tool for the analysis of Copaifera oleoresin and its products. (AU)

• Processo FAPESP: 14/03092-6 - Validação química e estudo da atividade antitumoral in vitro da oleorresina e dos principais metabólitos secundários de Copaifera multijuga
• Beneficiário: Luiza Junqueira Carneiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/01232-0 - Oleorresinas de Copaifera: desenvolvimento e validação de metodologia analítica por CLAE-DAD e estudo fitoquímico e antitumoral in vitro dos principais metabólitos secundários isolados
• Beneficiário: Luiza Junqueira Carneiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto