White adipose tissue IFN-gamma expression and signalling along the progression of rodent cancer cachexia

Autor(es)

Yamashita, Alex Shimura ; das Neves, Rodrigo Xavier ; Rosa-Neto, Jose Cesar ; Lira, Fabio dos Santos ; Batista, Miguel Luis, Jr. ; Alcantara, Paulo Sergio ; Otoch, Jose Pinhata ; Seelaender, Marilia

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 89, p. 5-pg., 2017-01-01.

Resumo

Cachexia is associated with increased morbidity and mortality in cancer. The White adipose tissue (WAT) synthesizes and releases several pro-inflammatory cytokines that play a role in cancer cachexia-related systemic inflammation. IFN-gamma is a pleiotropic cytokine that regulates several immune and metabolic functions. To assess whether IFN-gamma signalling in different WAT pads is modified along cancer-cachexia progression, we evaluated IFN-gamma receptors expression (IFNGR1 and IFNGR2) and IFN-gamma protein expression in a rodent model of cachexia (7, 10, and 14 days after tumour implantation). IFN-gamma protein expression was heterogeneously modulated in WAT, with increases in the mesenteric pad and decreased levels in the retroperitoneal depot along cachexia progression. Ifngr1 was up-regulated 7 days after tumour cell injection in mesenteric and epididymal WAT, but the retroperitoneal depot showed reduced Ifngr1 gene expression. Ifngr2 gene expression was increased 7 and 14 days after tumour inoculation in mesenteric WAT. The results provide evidence that changes in IFN-gamma expression and signalling may be perceived at stages preceding refractory cachexia, and therefore, might be employed as a means to assess the early stage of the syndrome. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/50079-0 - Inflamação sistêmica em pacientes com caquexia associada ao câncer: mecanismos e estratégias terapêuticas, uma abordagem em medicina translacional
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Modalidade de apoio: Auxílio à Pesquisa - Temático