Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries

Autor(es)

Picco, Daniele C. R. ; Marangoni-Lopes, Lenita ; Parisotto, Thais M. ; Mattos-Graner, Renata ; Nobre-dos-Santos, Marines

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 11, p. 9-pg., 2019-06-01.

Resumo

This study investigated pH, activity and concentration of carbonic anhydrase VI (CA VI) in dental biofilm of caries and caries-free children of 7-9 years old. Seventy-four children were selected and divided into two groups. The caries diagnosis was performed according to the WHO criteria, including the early caries lesion. After biofilm collection and pH determination, CA VI concentration and activity were determined by ELISA and Zimography respectively. The data were submitted to a Mann-Whitney test and to Pearson and Spearman correlation analyses. Means and standard deviations of dental caries for the caries group were of 3.162 +/- 1.385. The biofilm pH was significantly higher in the caries-free group. The CA VI activity was significantly higher in biofilm of children with caries. The CA VI concentration was significantly higher in biofilm of caries-free children. In caries-free children, there was a moderate negative correlation between CA VI activity and concentration in dental biofilm as well as between pH and CA VI activity. A negative correlation between biofilm pH and CA VI concentration was found in the caries group. In conclusion, CA VI was shown to be more active in the biofilm of school children with caries in order to contribute to neutralization of biofilm acid. (AU)

• Processo FAPESP: 12/02886-3 - Análise da concentração e atividade da anidrase carbônica VI (CA VI) na saliva e biofilme de crianças em idade escolar.
• Beneficiário: Daniele de Cassia Rodrigues Picco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/15834-1 - Análise da concentração e atividade da anidrase carbônica VI (AC VI) na saliva e biofilme na dentição decídua e sua relação com a cárie dental: estudo longitudinal em crianças de 3 a 6 anos e transverso em crianças de 7 a 9 anos
• Beneficiário: Marines Nobre dos Santos Uchôa
• Modalidade de apoio: Auxílio à Pesquisa - Regular