Chromosomal Evolution and Evolutionary Relationships of Lebiasina Species (Characiformes, Lebiasinidae)

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Cavalcante Sassi, Francisco de Menezes ; de Oliveira, Ezequiel Aguiar ; Carlos Bertollo, Luiz Antonio ; Nirchio, Mauro ; Hatanaka, Terumi ; Ferreira Marinho, Manoela Maria ; Moreira-Filho, Orlando ; Aroutiounian, Rouben ; Liehr, Thomas ; Al-Rikabi, Ahmed B. H. ; Cioffi, Marcelo de Bello

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 12, p. 17-pg., 2019-06-02.

Resumo

We present the first cytogenetic data for Lebiasina bimaculata and L. melanoguttata with the aim of (1) investigating evolutionary events within Lebiasina and their relationships with other Lebiasinidae genera and (2) checking the evolutionary relationships between Lebiasinidae and Ctenoluciidae. Both species have a diploid number 2n = 36 with similar karyotypes and microsatellite distribution patterns but present contrasting C-positive heterochromatin and CMA(3)(+) banding patterns. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the ribosomal DNA sites as the only GC-rich (CMA(3)(+)) regions, while L. melanoguttata shows evidence of a clear intercalated CMA(3)(+) banding pattern. In addition, the multiple 5S and 18S rDNA sites in L. melanogutatta contrast with single sites present in L. bimaculata. Comparative genomic hybridization (CGH) experiments also revealed a high level of genomic differentiation between both species. A polymorphic state of a conspicuous C-positive, CMA(3)(+), and (CGG)n band was found only to occur in L. bimaculata females, and its possible relationship with a nascent sex chromosome system is discussed. Whole chromosome painting (WCP) and CGH experiments indicate that the Lebiasina species examined and Boulengerella maculata share similar chromosomal sequences, thus supporting the relatedness between them and the evolutionary relationships between the Lebiasinidae and Ctenoluciidae families. (AU)

• Processo FAPESP: 18/24235-0 - Evolução cariotípica e de cromossomos sexuais em peixes neotropicais. parte iii.
• Beneficiário: Luiz Antonio Carlos Bertollo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/22033-1 - Uma abordagem intercontinental para a investigação da evolução cromossômica, diversidade genética e biogeografia na ordem Osteoglossiformes (Teleostei: Osteoglossomorpha). Parte III.
• Beneficiário: Marcelo de Bello Cioffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/09321-5 - Characiformes miniaturas (Teleostei: Ostariophysi): evolução, diversidade e biogeografia
• Beneficiário: Manoela Maria Marinho Koh
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores