| Texto completo | |
| Autor(es): |
Bosio Meneses, Claudia Caroline
;
Olivi, Lucas Tofanello
;
Carvalho, Ceci Nunes
;
Gavini, Giulio
;
Sipert, Carla Renata
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF ENDODONTICS; v. 46, n. 9, p. 5-pg., 2020-09-01. |
| Resumo | |
Introduction: The outcome of root canal obturation might be affected by the chemical components of the chosen filling materials. Niobium phosphate glass-based gutta-percha (GNB) was proposed as a biomaterial-based obturation point. This study aimed to investigate the cytotoxic and cell modulation effects of GNB points on human periodontal ligament fibroblasts (PDLFs) in vitro. Methods: Human PDLFs were cultured for the assays. Extracts of regular gutta-percha (GP) points and GNB were obtained, serially diluted (1:5, 1:10, and 1:25), and used to stimulate PDLFs. A cell viability assay was performed using alamarBlue reagent (Molecular Probes, Waltham, MA), and reverse transcription quantitative polymerase chain reaction was used to assess the gene expression for collagen type I and cementum protein 1. One-way analysis of variance followed by the Tukey post hoc test was performed (P < .05). Results: Regular GP reduced cell viability only in pure extracts, whereas GNB exhibited cytotoxicity to PDLFs in pure extracts as well as 1/5 and 1/10 dilutions. The gene expression of collagen type I was down-regulated only in the GNB group (P < .05). The expression of cementum protein 1 remained unaltered by both tested materials. Conclusions: The addition of niobium phosphate glass to GP points increased cytotoxicity, affecting PDLF viability and partially disturbing physiological cell function. (AU) | |
| Processo FAPESP: | 12/18220-4 - Efeito do compósito e do vidro niobofosfato bioativo na formação de biofilme |
| Beneficiário: | Ceci Nunes Carvalho |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 17/00643-0 - Efeito da guta-percha sobre a biomodulação in vitro de fibroblastos do ligamento periodontal |
| Beneficiário: | Lucas Tofanello Olivi |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/01737-8 - Papel de endocanabinóides na modulação de células de papila apical humana in vitro |
| Beneficiário: | Claudia Caroline Bosio Meneses |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/13944-5 - Papel de mediadores inflamatórios na modulação de células de papila apical humana in vitro |
| Beneficiário: | Carla Renata Sipert |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |