Impact of biomineralization on resin/biomineralized dentin bond longevity in a minimally invasive approach: An "in vitro" 18-month follow-up

Autor(es)

Moreira, Kelly Maria ; Bertassoni, Luiz Eduardo ; Davies, Robert Phill ; Joia, Felipe ; Hofling, Jose Francisco ; Nascimento, Fabio Duprat ; Puppin-Rontani, Regina Maria

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 37, n. 5, p. 14-pg., 2021-04-17.

Resumo

Objectives. To determine the impact of treating caries-affected dentin (CAD) with: 0.2% sodium fluoride (NaF), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP/MI Paste(TM)) or peptide P-11-4 (Curodont(TM) Repair) on the longevity of resin/CAD interface at storage times of 24-h, 6-and 18-month. Methods. 255 caries-free third molars were used, and CAD was produced by a biological method. The teeth were randomly distributed into: G1-Sound dentin (SD); G2-CAD; G3 CAD + 0.2% NaF (CAD/NaF); G4-CAD + CPP-ACP (CAD/ACP); G5-CAD + Curodont(TM) Repair (CAD/P-11-4). The Filtek Z350 composite resin block was bonded to dentin using Adper(TM) Single 2 (4 mm/height). Resin/dentin blocks were stored in a solution of Simulated Body Fluid at 37 degrees C, pressures were modified to simulate natural pulpal pressures. Specimens were investigated by microtensile bond strength (mu TBS) (n = 8), Scanning Electron Microscopy (to assess the failure mode) (n = 8), nanoinfiltration (to assess the interface sealing) (n = 3), in situ zymography (to assess the gelatinolytic activity) (n = 3) and micro-computed microtomography (mu-CT) (to assess the mineralization) (n = 3). Data from mu TBS, mu,-CT and, nanoinfiltration and hybrid layer formation/degradation were submitted to two-way ANOVA and Tukey tests, and failure patterns and in situ zymography to Kruskal-Wallis and Dunn tests (a = 5%). Results. The highest mineral density change by mu-CT, smallest silver nitrate infiltration and proteolytic activity in the adhesive layer were obtained significantly for the groups SD, CAD/ACP and CAD/P-11-4, with most mixed fractures at 18-month (p < 0.001). CAD/NaF showed significantly similar values to CAD, CAD and CAD/NaF which presented a high percentage of adhesive fracture (p < 0.001) at all time periods. Significance. Treating caries-affected dentin with remineralizing agents CPP-ACP and Curodont(TM) Repair, has the potential to be a clinically relevant treatment protocol to increase the longevity of adhesive restorations. (c) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/24927-1 - Impacto de agentes remineralizadores na longevidade da união resina/dentina afetada por cárie: resistência da união e nanoinfiltração
• Beneficiário: Kelly Maria Silva Moreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado