Glycogen synthase kinase 3 inhibition enhances mineral nodule formation by cementoblasts in vitro

Autor(es)

Henrique ABDALLA ^[1] ; Rafael STORINO ^[2] ; Amanda BANDEIRA ^[3] ; Lucas TEIXEIRA ^[4] ; Ana MILLÁS ^[5] ; Paulo LISBOA-FILHO ^[6] ; Kamila KANTOVITZ ^[7] ; Francisco NOCITI JUNIOR ^[8]

Afiliação do(s) autor(es)

^[1] Faculdade São Leopoldo Mandic. School of Dentistry. Department of Research - Brasil

^[2] Faculdade São Leopoldo Mandic. School of Dentistry. Department of Research - Brasil

^[3] Universidade José do Rosário Vellano University. School of Dentistry. Department of Periodontics - Brasil

^[4] Faculdade São Leopoldo Mandic. School of Dentistry. Department of Research - Brasil

^[5] Empresa de Biotecnologia e Soluções 3D. Department of Innovation - Brasil

^[6] Universidade Estadual Paulista. School of Sciences. Department of Physics and Meteorology - Brasil

^[7] Faculdade São Leopoldo Mandic. School of Dentistry. Department of Research - Brasil

^[8] Faculdade São Leopoldo Mandic. School of Dentistry. Department of Research - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 37, 2023-11-10.

Resumo

Abstract This study aimed to investigate whether GSK-3 inhibition (CHIR99021) effectively promoted mineralization by cementoblasts (OCCM-30). OCCM-30 cells were used and treated with different concentrations of CHIR99021 (2.5, 5, and 10 mM). Experiments included proliferation and viability, cellular metabolic activity, gene expression, and mineral nodule formation by Xylene Orange at the experimental time points. In general, CHIR99021 did not significantly affect OCCM-30 viability and cell metabolism (MTT assay) (p > 0.05), but increased OCCM-30 proliferation at 2.5 mM on days 2 and 4 (p < 0.05). Data analysis further showed that inhibition of GSK-3 resulted in increased transcript levels of Axin2 in OCCM-30 cells starting as early as 4 h, and regulated the expression of key bone markers including alkaline phosphatase (Alp), runt-related transcription factor 2 (Runx-2), osteocalcin (Ocn), and osterix (Osx). In addition, CHIR99021 led to an enhanced mineral nodule formation in vitro under both osteogenic and non-osteogenic conditions as early as 5 days after treatment. Altogether, the results of the current study suggest that inhibition of GSK-3 has the potential to promote cementoblast differentiation leading to increased mineral deposition in vitro. (AU)

• Processo FAPESP: 19/05274-8 - Bioimpressão 3D de enxertos vasculares e as biotintas utilizadas na aplicação
• Beneficiário: Ana Luíza Garcia Millás Massaguer
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 19/04276-7 - O uso de microagulhas revestíveis com fármacos para o controle de da dor e inflamação
• Beneficiário: Henrique Ballassini Abdalla
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado