Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms

Autor(es)

Adaias Oliveira Matos ; Valentim Adelino Ricardo Barão ^[2] ; Richard Lee Gregory ^[3]

• Tipo de documento: Artigo Científico
• Fonte: Braz. J. Oral Sci.; v. 22, 2023-12-01.

Resumo

Aim: The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides. (AU)

• Processo FAPESP: 17/21894-0 - Potencial antimicrobiano e de biocompatibilidade de filmes de hexametildisiloxano incorporados a clorhexidina em superfície de titânio usando um modelo de epitélio oral gengival co-infectado reconstituído in vitro
• Beneficiário: Adaias Oliveira Matos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado