Genetic variability of Leishmania (Leishmania) infantum causing human visceral leishmaniasis in the Southeastern Brazil

Autor(es)

Vinicius Alves Lima ; Renata Elen Costa Silva ; Luiz Henrique Moraes Caetano Camargo ^[3] ; Roberto Mitsuyoshi Hiramoto ^[4] ; Elcio de Souza Leal ^[5] ; Lucia Maria Almeida Braz ^[6] ; José Angelo Lauletta Lindoso

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 65, 2023-10-20.

Resumo

ABSTRACT Leishmania infantum is a protozoan that causes visceral leishmaniasis (VL) in the Americas and some regions of Europe. The disease is mainly characterized by hepatosplenomegaly and fever, and can be fatal. Factors related to the host and parasite can contribute to the transmission of Leishmania and the clinical outcome. The intraspecific genetic variability of L. infantum strains may be one of these factors. In this study, we evaluated the genetic variability of L. infantum obtained from bone marrow smear slides from patients in the Sao Paulo State, Brazil. For this, the minicircle of the kDNA hypervariable region was used as target by Sanger sequencing. By analyzing the similarity of the nucleotides and the maximum likelihood tree (Fasttree), we observed a high similarity (98%) among samples. Moreover, we identified four different profiles of L. infantum. In conclusion, L. infantum strains from Sao Paulo State, Brazil, showed low diversity measured by minicircle of the kDNA hypervariable region. (AU)

• Processo FAPESP: 18/05758-2 - Análise de polimorfismos da Leishmania (L.) infantum chagasi, por meio da técnica de microssatélites, em amostras de medula óssea de pacientes do estado de São Paulo, com leishmaniose visceral
• Beneficiário: Vinícius Alves de Lima
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/03165-9 - Coinfecção Leishmania/HIV-AIDS: Avaliação da prevalência da coinfecção e avaliação do perfil genotípico de Leishmania (Leishmania) infantum chagasi.
• Beneficiário: José Angelo Lauletta Lindoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular