| Texto completo | |
| Autor(es): |
Igor Henrique Cerqueira
[1]
;
Maria Julia Mieli
[2]
;
Anna Karla dos Santos Pereira
[3]
;
Pedro Paulo Corbi
[4]
;
Flavia Aparecida Resende
[5]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Universidade de Araraquara (UNIARA). Departamento de Ciências Biológicas e da Saúde - Brasil
[2] Universidade de Araraquara (UNIARA). Departamento de Ciências Biológicas e da Saúde - Brasil
[3] Universidade Federal do Tocantins (UFT) - Brasil
[4] Universidade de Campinas (UNICAMP). Instituto de Química - Brasil
[5] Universidade de Araraquara (UNIARA). Departamento de Ciências Biológicas e da Saúde - Brasil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Química Nova; v. 47, n. 6 2024-03-11. |
| Resumo | |
Silver(I) complexes of amantadine (atdH), memantine (mtnH) and rimantadine (rtdH), named Ag-atd, Ag-mtn and Ag-rtd, respectively, were recently synthesized and described in the literature as promising antibacterial agents. In the present study, the cytotoxicity of such complexes was evaluated against cultures of primary epidermal keratinocytes (HaCaT) and murine melanoma tumor cells (B16-F10), and mutagenicity was studied by the Ames test to investigate their abilities to induce gene mutations. The Ames test was performed using Salmonella Typhimurium strains (TA98, TA100, TA102 and TA97a) capable of detecting frameshift and base pair substitution gene mutations, in experiments with and without metabolic activation (microsomal fraction S9). This study revealed significant cytotoxic activity against tumor cells and selectivity of Ag-atd and Ag-rtd complexes when compared to non-tumor human keratinocyte cells. Moreover, the Ag(I) complexes did not induce a significant growth in the number of revertant colonies when comparing with the negative control, both in the experiments without and with metabolic activation, indicating absence of mutagenic activity. The results are encouraging and collaborate in the genotoxicological investigations necessary for understanding the interaction and ability of the silver complexes to induce mutations and contribute to ensure their uses as future antibacterial or antitumor agents. (AU) | |
| Processo FAPESP: | 17/16278-9 - Estudo do potencial genotoxicológico e da permeabilidade em células Caco-2 de complexos metálicos com promissoras atividades biológicas |
| Beneficiário: | Flávia Aparecida Resende Nogueira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 21/08717-8 - Síntese de complexos de cobre, prata e ouro envolvendo análogos de nucleotídeos na busca por novas alternativas para o tratamento do câncer de ovário |
| Beneficiário: | Pedro Paulo Corbi |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 21/10265-8 - Centro de Inovação Teranóstica em Câncer (CancerThera) |
| Beneficiário: | Carmino Antonio de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |