| Full text | |
| Author(s): |
Igor Henrique Cerqueira
[1]
;
Maria Julia Mieli
[2]
;
Anna Karla dos Santos Pereira
[3]
;
Pedro Paulo Corbi
[4]
;
Flavia Aparecida Resende
[5]
Total Authors: 5
|
| Affiliation: | [1] Universidade de Araraquara (UNIARA). Departamento de Ciências Biológicas e da Saúde - Brasil
[2] Universidade de Araraquara (UNIARA). Departamento de Ciências Biológicas e da Saúde - Brasil
[3] Universidade Federal do Tocantins (UFT) - Brasil
[4] Universidade de Campinas (UNICAMP). Instituto de Química - Brasil
[5] Universidade de Araraquara (UNIARA). Departamento de Ciências Biológicas e da Saúde - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Química Nova; v. 47, n. 6 2024-03-11. |
| Abstract | |
Silver(I) complexes of amantadine (atdH), memantine (mtnH) and rimantadine (rtdH), named Ag-atd, Ag-mtn and Ag-rtd, respectively, were recently synthesized and described in the literature as promising antibacterial agents. In the present study, the cytotoxicity of such complexes was evaluated against cultures of primary epidermal keratinocytes (HaCaT) and murine melanoma tumor cells (B16-F10), and mutagenicity was studied by the Ames test to investigate their abilities to induce gene mutations. The Ames test was performed using Salmonella Typhimurium strains (TA98, TA100, TA102 and TA97a) capable of detecting frameshift and base pair substitution gene mutations, in experiments with and without metabolic activation (microsomal fraction S9). This study revealed significant cytotoxic activity against tumor cells and selectivity of Ag-atd and Ag-rtd complexes when compared to non-tumor human keratinocyte cells. Moreover, the Ag(I) complexes did not induce a significant growth in the number of revertant colonies when comparing with the negative control, both in the experiments without and with metabolic activation, indicating absence of mutagenic activity. The results are encouraging and collaborate in the genotoxicological investigations necessary for understanding the interaction and ability of the silver complexes to induce mutations and contribute to ensure their uses as future antibacterial or antitumor agents. (AU) | |
| FAPESP's process: | 21/10265-8 - Cancer Theranostics Innovation Center (CancerThera) |
| Grantee: | Carmino Antonio de Souza |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 17/16278-9 - Study of the genotoxicological potential and permeation assay with Caco-2 cells of metallic complexes with promising biological activities |
| Grantee: | Flávia Aparecida Resende Nogueira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/08717-8 - Synthesis of copper, silver and gold complexes involving nucleotide analogues in the search of new alternatives for the treatment of ovarian cancer |
| Grantee: | Pedro Paulo Corbi |
| Support Opportunities: | Regular Research Grants |