Time-efficient germ cell transplantation from goldfish (Carassius auratus) into adult common carp (Cyprinus carpio)

Autor(es)

Angel Andreas Arias Vigoya ; Daniel Fernandes da Costa ^[2] ; Marcos Antônio de Oliveira ; Arno Juliano Butzge ^[4] ; Ivana Felipe Rosa ^[5] ; Lucas Benites Doretto ; Emanuel Ricardo Monteiro Martinez ^[7] ; Melanie Digmayer ^[8] ; Rafael Henrique Nóbrega ^[9]

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction; v. 21, n. 1 2024-02-12.

Resumo

Abstract Germ cell transplantation in fish is a promising technique for surrogate broodstock parents with broader application in aquaculture and conserving endangered and valuable genetic resources. Herein, we describe the establishment of an intrapapillary xenogeneic transplant of germ cells from sexually mature goldfish (C. auratus) males into common carp (C. carpio) males cytoablated with a thermochemical treatment (two doses of busulfan at 40 mg/kg at 35°C). To analyze the presence and development of donor germ cells in recipient testes, donor germ cells were labeled with PKH26, a fluorescent cell membrane dye, before transplantation. Our results demonstrated that thermochemical treatment caused effective spermatogenesis suppression and pronounced germ cell loss. Moreover, transplanted spermatogonial cells were able to colonize the recipients’ testes, resume spermatogenesis, and generate spermatozoa within eight weeks after germ cell transplantation. These findings suggested that recipient testes provided suitable conditions for the survival, colonization, proliferation, and differentiation of donor spermatogonia from a related species. This study indicated that recipients’ testes exhibited a high degree of plasticity to accept and support xenogeneic donor germ cells, which were able to form sperm in a short time frame. This approach has significant implications for assisted animal reproduction, biotechnology, conservation, and the production of valuable genetic resources and endangered fish species. (AU)

• Processo FAPESP: 21/06742-5 - Novos insights sobre sinais gonadais (fatores de crescimento e lncRNAs) que regulam a maturação gonadal em peixes: conhecimento básico e desenvolvimento de estratégias biotecnológicas para retardar a puberdade
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 12/00423-6 - Regulação parácrina e molecular do nicho de espermatogônias tronco em zebrafish (Danio rerio) e uso da carpa comum (Cyprinus carpio) como modelo para atrasar a diferenciação espermatogonial durante a puberdade
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/07620-7 - Banco de espermatogônias tronco em peixes para conservação do genótipo de espécies ameaçadas e de interesse econômico
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores