Fast multispectral imaging analysis as phenotyping platform: Effect of titanium dioxide nanoparticles on microalgae physiology

Autor(es)

Lavandosque, L. L. ; Mastrangelo, C. B. ; Carvalho, H. W. P. C. ; Guimara, R. R. ; Araki, K. ; Winck, F. V.

• Tipo de documento: Artigo Científico
• Fonte: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS; v. 78, p. 12-pg., 2024-02-16.

Resumo

Several types of nanoparticles have been developed and explored in agricultural applications and their environmental impact evaluated. However, no such study was carried out using fully dispersible ultrasmall titanium dioxide nanoparticles (us-TiO2). Therefore, we investigate the effects of fully dispersible 2.5 nm large us-TiO2 nanoparticles on the cellular metabolism of the microalgae Chlamydomonas reinhardtii using multispectral imaging analysis as a platform for the evaluation of their physiological responses. Our results indicate that us-TiO2 modulates the microalgae cell growth in a concentration-dependent manner. X-ray fluorescence analysis showed that the us-TiO2 strongly interacts with the cells in relatively low and constant amounts in the range of 0.05 g center dot L-1 to 1.5 g center dot L-1. Most nanoparticles were found on the cell surface, surrounding the microalgae cells dispersed in TAP medium, indicating a low trend of internalization. We further applied multispectral imaging analyses toward fast phenotyping of the alterations in the cell's physiology. Furthermore, higher concentrations of us-TiO2 (similar to 1 g center dot L-1) positively affect the microalgae photosynthetic activity and growth. Thus, our results indicated the capacity to detect variations in cell physiology and indicated a possible role of us-TiO2 in modulating positively microalgae photosynthesis and growth in a concentration-dependent way. The mechanisms controlling such responses must be further investigated. (AU)

• Processo FAPESP: 18/03793-5 - EMU concedido no processo 2017/15220-7: sistema de imagem SeedReporter câmera spectral & colour
• Beneficiário: Clíssia Barboza Mastrangelo
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 16/06601-4 - Análise integrativa de sistemas para o estudo de redes de regulação da expressão gênica relacionadas à produção de biomassa em microalgas
• Beneficiário: Flavia Vischi Winck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 22/15431-6 - Análise proteômica dos efeitos da inibição de função Arginase-like na regulação da síntese de lipídios neutros em microalgas
• Beneficiário: Leandro Luis Lavandosque
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica