| Texto completo | |
| Autor(es): Mostrar menos - |
Silva, Bruna Rafaela dos Santos
;
Sidarta-Oliveira, Davi
;
Morari, Joseane
;
Bombassaro, Bruna
;
Jara, Carlos Poblete
;
Simeoni, Camila Lopes
;
Parise, Pierina Lorencini
;
Proenca-Modena, Jose Luiz
;
Velloso, Licio A.
;
Velander, William H.
;
Araujo, Eliana P.
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Viruses-Basel; v. 16, n. 7, p. 17-pg., 2024-07-01. |
| Resumo | |
SARS-CoV-2 can induce vascular dysfunction and thrombotic events in patients with severe COVID-19; however, the cellular and molecular mechanisms behind these effects remain largely unknown. In this study, we used a combination of experimental and in silico approaches to investigate the role of PC in vascular and thrombotic events in COVID-19. Single-cell RNA-sequencing data from patients with COVID-19 and healthy subjects were obtained from the publicly available Gene Expression Omnibus (GEO) repository. In addition, HUVECs were treated with inactive protein C before exposure to SARS-CoV-2 infection or a severe COVID-19 serum. An RT-qPCR array containing 84 related genes was used, and the candidate genes obtained were evaluated. Activated protein C levels were measured using an ELISA kit. We identified at the single-cell level the expression of several pro-inflammatory and pro-coagulation genes in endothelial cells from the patients with COVID-19. Furthermore, we demonstrated that exposure to SARS-CoV-2 promoted transcriptional changes in HUVECs that were partly reversed by the activated protein C pretreatment. We also observed that the serum of severe COVID-19 had a significant amount of activated protein C that could protect endothelial cells from serum-induced activation. In conclusion, activated protein C protects endothelial cells from pro-inflammatory and pro-coagulant effects during exposure to the SARS-CoV-2 virus. (AU) | |
| Processo FAPESP: | 20/04558-0 - Caracterização de fatores de risco intrínsecos e o desenvolvimento de novas alternativas de diagnóstico e tratamento para COVID-19 |
| Beneficiário: | José Luiz Proença Módena |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas |
| Beneficiário: | Licio Augusto Velloso |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |