Involvement of GDF-9, leptin, and IGF1 receptors associated with adipose tissue transplantation on fertility restoration in obese anovulatory mice

Autor(es)

Pereira, Jr., Melquiades ^[1] ; Vidotti, Daniela Berguio ^[1] ; Borra, Ricardo Carneiro ^[2] ; Simoes, Manuel De Jesus ^[3] ; Cotrim Guerreiro Da Silva, Ismael Dale ^[1] ; Haidar, Mauro Abi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Gynecol, BR-04039032 Sao Paulo - Brazil

^[2] Sao Leopoldo Mand, Postgrad Program, Campinas, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04039032 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Gynecological Endocrinology; v. 27, n. 10, p. 759-766, OCT 2011.
• Citações Web of Science: 4

Resumo

The aim was to analyze the effect of adipose tissue transplantation on growth differentiation factor-9 (GDF-9), insulin growth factor 1 receptor (IGF1R), and leptin receptor (LEPR) protein expression in ovaries of obese anovulatory mice. Leptin-deficient female (ob/ob) and wild-type mice were divided into untreated ob/ob mice and gonadal white adipose tissue transplanted ob/ob mice, with evaluation after 7, 15, and 45 days and compared to control wild-type mice. The corporal weight and glycemia levels increased in the obese group concomitant with polymicrocyst formation and abundant estrone, mimicking anovulatory disease. In the treated group after 45 days, glycemia, weight, ovarian size, and number of follicles were decreased and corpora lutea were decreased. The analysis of GDF-9 revealed that, whereas control ovaries presented follicular localization, the obese ovary lacked this protein. On the other hand, obese ovaries showed elevated expression of IGF1R that was normalized after the transplantation. Finally, LEPR was reduced in obese ovaries, and adipose tissue transplantation was efficient in returning it to normal levels. In conclusion, the adipose tissue transplantation, especially after 45 days, seems to stimulate ovulation, supported by the fact that several proteins involved in ovulation returned to basal levels. (AU)

• Processo FAPESP: 07/55629-0 - Influencia da hiperinsulinemia na sindrome dos ovarios policisticos: analise genetica e proteomica.
• Beneficiário: Daniela Berguio Vidotti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado