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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Involvement of GDF-9, leptin, and IGF1 receptors associated with adipose tissue transplantation on fertility restoration in obese anovulatory mice

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Author(s):
Pereira, Jr., Melquiades [1] ; Vidotti, Daniela Berguio [1] ; Borra, Ricardo Carneiro [2] ; Simoes, Manuel De Jesus [3] ; Cotrim Guerreiro Da Silva, Ismael Dale [1] ; Haidar, Mauro Abi [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Dept Gynecol, BR-04039032 Sao Paulo - Brazil
[2] Sao Leopoldo Mand, Postgrad Program, Campinas, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04039032 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Gynecological Endocrinology; v. 27, n. 10, p. 759-766, OCT 2011.
Web of Science Citations: 4
Abstract

The aim was to analyze the effect of adipose tissue transplantation on growth differentiation factor-9 (GDF-9), insulin growth factor 1 receptor (IGF1R), and leptin receptor (LEPR) protein expression in ovaries of obese anovulatory mice. Leptin-deficient female (ob/ob) and wild-type mice were divided into untreated ob/ob mice and gonadal white adipose tissue transplanted ob/ob mice, with evaluation after 7, 15, and 45 days and compared to control wild-type mice. The corporal weight and glycemia levels increased in the obese group concomitant with polymicrocyst formation and abundant estrone, mimicking anovulatory disease. In the treated group after 45 days, glycemia, weight, ovarian size, and number of follicles were decreased and corpora lutea were decreased. The analysis of GDF-9 revealed that, whereas control ovaries presented follicular localization, the obese ovary lacked this protein. On the other hand, obese ovaries showed elevated expression of IGF1R that was normalized after the transplantation. Finally, LEPR was reduced in obese ovaries, and adipose tissue transplantation was efficient in returning it to normal levels. In conclusion, the adipose tissue transplantation, especially after 45 days, seems to stimulate ovulation, supported by the fact that several proteins involved in ovulation returned to basal levels. (AU)