Interactions between TLR2, TLR4, and mannose receptors with gp43 from Paracoccidioides brasiliensis induce cytokine production by human monocytes

Autor(es)

Nakaira-Takahagi, Erika ^[1] ; Golim, Marjorie A. ^[2] ; Bannwart, Camila F. ^[1] ; Puccia, Rosana ^[3] ; Peracoli, Maria T. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Microbiol & Imunol, Inst Biociencias, BR-18618970 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, UNESP, Hemoctr, Fac Med, BR-18618970 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Univ Fed Sao Paulo, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 49, n. 7, p. 694-703, OCT 2011.
• Citações Web of Science: 18

Resumo

The glycoprotein gp43 is an immunodominant antigen secreted by Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis. The present study evaluated whether gp43 can interact with toll-like (TLR2, TLR4) and mannose (MR) receptors on the surface of human monocytes, and how that affects their expression and cytokine production. Monocytes were incubated with or without monoclonal antibodies anti-TLR2, anti-TLR4, or anti-MR, individually or in combination, prior to the addition of gp43. The gp43 binding to monocyte surface, as well as expression of TLR2, TLR4, and MRs were analyzed by flow cytometry, while production of TNF-alpha and IL-10 was monitored by ELISA. The results suggested that gp43 binds to TLR2, TLR4, and MR receptors, with TLR2 and MR having the strongest effect. All three receptors influenced the production of IL-10, while TNF-alpha production was associated with expression of TLR4 and MR. The modulatory effect of gp43 was demonstrated by high levels of TLR4 expression associated with increased production of TNF-alpha after 4 h of culture. Alternatively, high levels of TLR2 expression, and elevated production of IL-10, were detected after 18 h. We showed that interaction between gp43 and monocytes may affect the innate immune response by modulating the expression of the pattern recognition receptors TLR2, TLR4 and MR, as well as production of pro- and anti-inflammatory cytokines. (AU)

• Processo FAPESP: 06/53366-9 - Envolvimento de receptores celulares tlr2, tlr4 e cd14 na producao in vitro de fator de necrose tumoral-alfa e interleucina-10 por monocitos humanos estimulados com paracoccidioides brasiliensis.
• Beneficiário: Erika Nakaira Takahagi
• Linha de fomento: Bolsas no Brasil - Doutorado