Differentiation of Ayahuasca Samples According to Preparation Mode and Botanical Varieties Using Metabolomics

Autor(es)

Matos, Taynara Simao ; Zandonadi, Flavia da Silva ; Silva, Alex Aparecido Rosini ; Soares, Stephanie Dias ; Lima, Adriana de Souza ; Pastore, Glaucia Maria ; Porcari, Andreia de Melo ; Sussulini, Alessandra

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PSYCHOACTIVE DRUGS; v. N/A, p. 10-pg., 2024-10-31.

Resumo

Ayahuasca is a brew traditionally prepared with a mixture of Psychotria viridis leaves and Banisteriopsis caapi vine and has demonstrated therapeutic properties for depression. Knowledge of the brew composition is important to improve the therapeutic potential and decrease side effects if ayahuasca becomes an option for refractory depression treatment. Ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) was applied to analyze 126 ayahuasca samples collected from different ayahuasqueiro groups and geographic origins. We were able to observe a differentiation in the metabolite composition of ayahuasca samples prepared by diverse ayahuasqueiro groups. These samples presented different antioxidant effects based on FRAP and ORAC assays. Exploratory statistical analysis demonstrated a trend of separating the samples according to the religious group. The most important identified compounds for differentiation of the brew prepared by distinct religious groups are glycosylated and/or phenolic compounds. The comparison based on the mode of ayahuasca preparation presented more variability than the comparison based on the botanical variety of B. caapi used. We conclude that ayahuasca samples prepared with "caupuri" or "tucunac & aacute;" separately exhibited differences in the analysis of L-glutamate and the metabolism of arginine and proline. This suggests that a possible variation in this pathway could explain the occurrence of swollen stem nodes in "caupuri," one of the B. caapi varieties. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/01800-2 - Explorando o potencial da cannabis no tratamento de doenças neurodegenerativas
• Beneficiário: Alessandra Sussulini
• Modalidade de apoio: Auxílio à Pesquisa - Regular