Influence of irradiated dentin, biofilm and different artificial saliva formulations on root dentin demineralization

Autor(es)

de Souza, Beatriz Martines ; Braga, Aline Silva ; Vertuan, Mariele ; Sassaki, Susan ; Araujo, Tamara Teodoro ; Santos, Paulo Sergio da Silva ; Buzalaf, Marilia Afonso Rabelo ; Magalhaes, Ana Carolina

• Tipo de documento: Artigo Científico
• Fonte: HELIYON; v. 10, n. 16, p. 10-pg., 2024-08-30.

Resumo

The aim of this study was evaluated the influence of radiation as well as of new formulations of artificial saliva on the development of root dentin lesions. Bovine root samples were divided into: irradiated (70 Gy) dentin or not; the type of biofilm (from irradiated patient-experimental or non-irradiated patient-control) and the type of artificial saliva (for the condition irradiated dentin/biofilm from irradiated patient): Control Artificial Saliva (inorganic); Control Saliva + 1 mg/ml hemoglobin; Control Saliva +0.1 mg/ml cystatin; Control Saliva + hemoglobin + cystatin; Bioextra (positive control) and deionized water (DiW, negative control) (n = 12/group). Biofilm was produced using human biofilm and McBain saliva (0.2 % of sucrose, 37(o) C and 5 % CO2); the treatments were done 1x/day, for 5 days. Colony-forming units (CFU) counting was performed; demineralization was quantified by transversal microradiography. Two-way ANOVA/Bonferroni or Sidak test for the comparison between biofilm x dentin and ANOVA/Tukey or Kruskal-Wallis/Dunn for comparing artificial saliva were done (p < 0.05). The type of biofilm had no influence on CFU and demineralization. Sound dentin under control biofilm presented the lowest Lactobacillus ssp. and Streptococcus mutans CFU and the lowest mean mineral loss (R) (25.6 +/- 2.2; 23.7 +/- 2.9 %) compared to irradiated dentin (26.1 +/- 2.8; 28.1 +/- 3.3, p < 0.004) for both types of biofilms (experimental and control, respectively). Bioextra was the only artificial saliva that reduced R (10.8 +/- 2.5 %) and Lesion Depth (LD) (35 +/- 15 mu m) compared to DiW (17.3 +/- 3.3 %, 81 +/- 18 mu m, p < 0.0001). Irradiation has impact on caries development; the experimental saliva were unable to reduce its occurrence. (AU)

• Processo FAPESP: 19/07241-0 - Efeito de formulações de saliva artificial e aplicação de fluoretos sobre o desenvolvimento de cárie radicular em modelo de biofilme microcosmo simulando pacientes submetidos à radiação de cabeça e pescoço
• Beneficiário: Beatriz Martines de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/21797-0 - Efeito de formulações de saliva artificial e aplicação de fluoretos sobre o desenvolvimento de cárie radicular em modelo de biofilme microcosmo simulando pacientes submetidos à radiação de cabeça e pescoço
• Beneficiário: Ana Carolina Magalhães
• Modalidade de apoio: Auxílio à Pesquisa - Regular