Disposable Pipette Extraction Followed by Direct MS/MS Analysis of Beta Amyloid Peptides (A β 38, A β 40, and A β 42) in Cerebrospinal Fluid Samples

Autor(es)

Grecco, Caroline F. ; Crevelin, Eduardo Jose ; Tumas, Vitor ; Hallak, Jaime Eduardo C. ; Queiroz, Maria Eugenia C.

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 36, n. 1, p. 10-pg., 2025-01-01.

Resumo

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by extracellular accumulation of amyloid-beta (A beta) peptides in the brain. This study demonstrates the direct association of the disposable pipette extraction (DPX) with tandem mass spectrometry (MS/MS) for the analysis of A beta peptides in cerebrospinal fluid (CSF) samples. Different parameters were optimized in order to improve detectability in the MS/MS including mobile phase percentage of ammonium hydroxide, mobile phase flow rate and acquisition mode. Also, this method used an electrospray ionization (ESI) low-flow probe and direct infusion of an organic solution in the MS/MS. The DPX-MS/MS method showed adequate linearity for determining A beta peptides in CSF-linearity ranged from 0.1 to 1.5 ng mL -1 . The coefficients of determination were higher than 0.99; the precision coefficient of variation (CV) ranged from 0.3 to 12.7%; and the accuracy relative standard deviation (RSD) ranged from -13.6 to 13.2%. (AU)

• Processo FAPESP: 20/00126-8 - Desenvolvimento de métodos de microextração hifenados à LC-MS/MS ou diretamente à MS/MS para a determinação de biomarcadores em amostras biológicas de pacientes ou de ratos com doenças neurodegenerativas
• Beneficiário: Maria Eugênia Queiroz Nassur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/04386-7 - Desenvolvimento de fase monolítica orgânica para determinação de peptídeos b-amilóide AB40 e AB42 em amostras de plasma de pacientes com a Doença de Alzheimer por UHPLC-MS/MS no modo column switching
• Beneficiário: Caroline Fernandes Grecco
• Modalidade de apoio: Bolsas no Brasil - Doutorado