Genomic Characterization of a Clinical NDM-1-Producing Klebsiella michiganensis from Brazil

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Yamada, Amanda Yaeko ; de Souza, Andreia Rodrigues ; Bertani, Amanda Maria de Jesus ; Campos, Karoline Rodrigues ; Sacchi, Claudio Tavares ; de Assis, Denise Brandao ; de Carvalho, Eneas ; Takagi, Elizabeth Harummyy ; Cunha, Marcos Paulo Vieira ; Tiba-Casas, Monique Ribeiro ; Camargo, Carlos Henrique

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 12, n. 7, p. 10-pg., 2024-07-01.

Resumo

Public health faces daily challenges due to increasing reports of pathogenic microorganisms with new antimicrobial resistance. Klebsiella michiganensis, an emerging pathogen, poses difficulty in its identification using conventional techniques. This study presents the first documented case of NDM-1-producing K. michiganensis in Brazil, identified as the new ST418. Initially, the isolate from a tracheal secretion was misidentified as K. oxytoca. However, accurate identification was achieved through ANI analyses. Whole-genome sequencing was conducted to characterize the genetic context of the resistance genes, to identify virulence factors, and to construct a phylogenetic tree. The bla(NDM-1) gene was found to be harbored on an IncFIB plasmid approximately 112 kb in length, which was transferable in conjugation assays. The detection of carbapenem resistance genes in this species highlights the importance of public health vigilance, as it may serve as a reservoir and disseminator of significant resistance genes. (AU)

• Processo FAPESP: 20/06157-2 - Epidemiologia genômica de enterobactérias produtoras da carbapenemase NDM em isolados clínicos brasileiros
• Beneficiário: Carlos Henrique Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/11794-7 - Estudo genômico e filogenético de cepas emergentes de Acinetobacter baumannii pertencentes ao Clone Internacional 2 durante o período de pandemia da COVID-19
• Beneficiário: Amanda Yaeko Yamada
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/50333-7 - Plano de desenvolvimento institucional em pesquisa do Instituto Adolfo Lutz (PDIp)
• Beneficiário: Carlos Henrique Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa
• Processo FAPESP: 18/21192-9 - EMU concedido no processo 2017/50333-7: MALDI-TOF
• Beneficiário: Carlos Henrique Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários