Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains

Autor(es)

Cardoso, Camila A. ; Araujo, Gabriela, V ; Sandoval, Carmen M. ; Nogueira, Paula M. ; Zuniga, Concepcion ; Sosa-Ochoa, Wilfredo H. ; Laurenti, Marcia D. ; Soares, Rodrigo P.

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 115, p. 6-pg., 2020-01-01.

Resumo

Although Leishmania infantum is well-known as the aethiological agent of visceral leishmaniasis (VL), in some Central American countries it may cause atypical non-ulcerated cutaneous leishmaniasis ( NUCL). However, the mechanisms favoring its establishment in the skin are still unknown. Lipophosphoglycan (LPG) is the major Leishmania multivirulence factor involved in parasite-host interaction. In the case of viscerotropic L. infantum, it causes an immunosuppression during the interaction with macrophages. Here, we investigated the biochemical and functional roles of LPGs from four dermotropic L. infantum strains from Honduras during in vitro interaction with murine macrophages. LPGs were extracted, purified and their repeat units analysed. They did not have side chains consisting of Gal(beta 1,4)Man(alpha 1)-PO4 common to all LPGs. Peritoneal macrophages from BALB/c and C57BL/6 were exposed to LPG for nitric oxide (NO) and cytokine (TNF-alpha and, IL-6) production. LPGs from dermotropic strains from Honduras triggered higher NO and cytokine levels compared to those from viscerotropic strains. In conclusion, LPGs from dermotropic strains are devoid of side-chains and exhibit high pro-inflammatory activity. (AU)

• Processo FAPESP: 18/04698-6 - Leishmaniose cutânea não ulcerada ou atípica causada pela Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras: caracterização imuno-histopatológica das lesões de pele com ênfase na participação das células apresentadoras de antígenos
• Beneficiário: Carmen Maria Sandoval Pacheco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/24834-9 - Avaliação da evolução da infecção experimental em hamster por parasitos isolados de lesão cutânea atípica e visceral humana causada por Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras
• Beneficiário: Gabriela Venicia Araujo Flores
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Temático