Optimization of the Enzymatic Hydrolysis of Blue Shark Skin

Autor(es)

Rodriguez-Diaz, Julio C. ^[1] ; Kurozawa, Louise E. ^[2] ; Netto, Flavia M. ^[3] ; Hubinger, Miriam D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Food Engn, Fac Food Engn, BR-13083862 Sao Paulo - Brazil

^[2] Rural Fed Univ Rio de Janeiro, Dept Food Technol, BR-23890000 Rio De Janeiro - Brazil

^[3] Univ Estadual Campinas, Dept Food & Nutr, Fac Food Engn, BR-13083862 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Food Science; v. 76, n. 7, p. C938-C949, SEP 2011.
• Citações Web of Science: 9

Resumo

Enzymatic hydrolysis of Blue shark skin using Protamex (TM) was evaluated seeking optimal process conditions. The influence of temperature (45 to 65 degrees C), pH (6.8 to 8), and enzyme/substrate ratio (E/S; 1% to 5%) on the responses of degree of hydrolysis and protein recovery were determined and process optimization was performed looking for maximum value of the responses. Optimum conditions were established (T = 51 degrees C, E/S = 4%, and pH = 7.1) and model validation was accomplished by triplicate. Under these conditions protein hydrolysates were prepared and characterized by their amino acid composition, peptide size distribution, and antioxidant capacity by ferric reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays. A degree of hydrolysis of 19.3% and protein recovery of 90.3% were obtained at optimal conditions. Chemical score indicated that the hydrolysate supplies minimal essential amino acid requirements for adults. Molecular weight of peptides on the hydrolysate was below 6.5 kDa. Enzymatic hydrolysis process was efficient for recovery of low molecular weight peptides with important nutritional content and antioxidant activity (FRAP = 12 mu mol eq. in FeSO(4)center dot 7H(2)O/g of protein, TEAC = 225.3 mu mol eq. in trolox/g of protein). (AU)

• Processo FAPESP: 09/54137-1 - EMUS: aquisição de um analisador de distribuição de tamanho de partículas, um medidor de potencial zeta, um spray dryer e um sistema de cromatografia líquida de ultra alta pressão / espectrômetro de massa
• Beneficiário: Miriam Dupas Hubinger
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 07/54520-4 - Estudo do processo de hidrolise enzimatica de carne de frango, microencapsulacao e secagem por spray dryer.
• Beneficiário: Miriam Dupas Hubinger
• Modalidade de apoio: Auxílio à Pesquisa - Regular