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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Optimization of the Enzymatic Hydrolysis of Blue Shark Skin

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Author(s):
Rodriguez-Diaz, Julio C. [1] ; Kurozawa, Louise E. [2] ; Netto, Flavia M. [3] ; Hubinger, Miriam D. [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Dept Food Engn, Fac Food Engn, BR-13083862 Sao Paulo - Brazil
[2] Rural Fed Univ Rio de Janeiro, Dept Food Technol, BR-23890000 Rio De Janeiro - Brazil
[3] Univ Estadual Campinas, Dept Food & Nutr, Fac Food Engn, BR-13083862 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Food Science; v. 76, n. 7, p. C938-C949, SEP 2011.
Web of Science Citations: 9
Abstract

Enzymatic hydrolysis of Blue shark skin using Protamex (TM) was evaluated seeking optimal process conditions. The influence of temperature (45 to 65 degrees C), pH (6.8 to 8), and enzyme/substrate ratio (E/S; 1% to 5%) on the responses of degree of hydrolysis and protein recovery were determined and process optimization was performed looking for maximum value of the responses. Optimum conditions were established (T = 51 degrees C, E/S = 4%, and pH = 7.1) and model validation was accomplished by triplicate. Under these conditions protein hydrolysates were prepared and characterized by their amino acid composition, peptide size distribution, and antioxidant capacity by ferric reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays. A degree of hydrolysis of 19.3% and protein recovery of 90.3% were obtained at optimal conditions. Chemical score indicated that the hydrolysate supplies minimal essential amino acid requirements for adults. Molecular weight of peptides on the hydrolysate was below 6.5 kDa. Enzymatic hydrolysis process was efficient for recovery of low molecular weight peptides with important nutritional content and antioxidant activity (FRAP = 12 mu mol eq. in FeSO(4)center dot 7H(2)O/g of protein, TEAC = 225.3 mu mol eq. in trolox/g of protein). (AU)

FAPESP's process: 09/54137-1 - Acquisition of a particle size/distribution analyzer, a zeta potential measurement system, a spray dryer, and an ultra-high-pressure liquid chromatograph/mass spectrometer
Grantee:Miriam Dupas Hubinger
Support Opportunities: Multi-user Equipment Program