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Development of optimized enzyme cocktails for hydrolysis of sugar cane bagasse

Grant number: 12/23223-2
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2013
Effective date (End): August 31, 2014
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Aline Carvalho da Costa
Grantee:Bianca Consorti Bussamra
Home Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:08/57873-8 - An integrated process for total bioethanol production and zero CO2 emission, AP.BIOEN.TEM


The high cost of lignocellulosic biomass conversion by enzymatic hydrolysis is a recurring problem that limits the production of bioethanol from this renewable and highly energetic source. Among the promising approaches to increase the efficiency of the hydrolysis reaction, may be mentioned the development of individually more efficient and stable enzymes through the use of genetic engineering tools and the development of well balanced artificial cocktails between different classes of enzymes and tailor made for biomass to be hydrolyzed. Thus, this project proposes the development of optimized enzyme cocktails based on the rational mixture of essential and accessory enzymes for the saccharification of pretreated sugar cane bagasse. The optimization of the enzymatic mixture composition will be performed by factorial design tools and response surface methodology. The evaluation of the synergism will be based on the micro enzymatic reactions to be validated in this study for sugar cane bagasse pretreated. It is hoped, with the development of this project, to contribute to the understanding of the enzymatic hydrolysis reaction of pretreated bagasse, to generate a more efficient and tailor made enzyme mixture for this biomass and to collect data for the development of a minimum cocktail for the saccharification of bagasse. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUSSAMRA, BIANCA CONSORTI; FREITAS, SINDELIA; DA COSTA, ALINE CARVALHO. Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail. Bioresource Technology, v. 187, p. 173-181, JUL 2015. Web of Science Citations: 16.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BUSSAMRA, Bianca Consorti. Improvement of enzymatic cocktails for sugar cane bagasse hydrolysis. 2014. Master's Dissertation - Universidade Estadual de Campinas. Faculdade de Engenharia Química.

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