In vitro and In vivo Anticancer Activity of Extracts, Fractions, and Eupomatenoid-5 Obtained from Piper regnellii Leaves

Autor(es)

Longato, Giovanna Barbarini ^{[1, 2]} ; Rizzo, Larissa Yokota ^{[1, 2]} ; de Oliveira Sousa, Ilza Maria ^[3] ; Tinti, Sirlene Valerio ^[1] ; Possenti, Ana ^[1] ; Figueira, Glyn Mara ^[4] ; Tasca Gois Ruiz, Ana Lucia ^[1] ; Foglio, Mary Ann ^[3] ; de Carvalho, Joao Ernesto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Div Farmacol & Toxicol, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr CP, Campinas, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Inst Biol, Programa Posgrad Biol Celular & Estrutural, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Div Fitoquim, CPQBA, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, CPQBA, Div Agrotecnol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Planta Medica; v. 77, n. 13, p. 1482-1488, SEP 2011.
• Citações Web of Science: 13

Resumo

Despite numerous studies with the Piper genus, there are no previous results reporting in vitro or in vivo Piper regnellii (Miq.) C. DC. var. regnellii anticancer activity. The aim of this study was to investigate P. regnellii in vitro and in vivo anticancer activity and further identify its active compounds. In vitro antiproliferative activity was evaluated in 8 human cancer cell lines: melanoma (UACC-62), breast (MCF7), kidney (786-0), lung (NCI-H460), prostate (PC-3), ovary (OVCAR-3), colon (HT29), and leukemia (K-562). Total growth inhibition (TGI) values were chosen to measure antiproliferative activity. Among the cell lines evaluated, eupomatenoid-5 demonstrated better in vitro antiproliferative activity towards prostate, ovary, kidney, and breast cancer cell lines. In vivo studies were carried out with Ehrlich solid tumor on Balb/C mice treated with 100, 300, and 1000 mg/kg of P. regnellii leaves dichloromethane crude extract (DCE), with 30 and 100 mg/kg of the active fraction (FRB), and with 30 mg/kg of eupomatenoid-5. The i.p. administration of DCE, FRB, and eupomatenoid-5 significantly inhibited tumor progression in comparison to control mice (saline). Therefore, this study showed that neolignans of Piper regnellii have promising anticancer activity. Further studies will be undertaken to determine the mechanism of action and toxicity of these compounds. (AU)

• Processo FAPESP: 08/50836-0 - Atividade anticancer de extratos brutos e fracoes ativas obtidos de piper regnellii.
• Beneficiário: Giovanna Barbarini Longato
• Modalidade de apoio: Bolsas no Brasil - Mestrado