| Texto completo | |
| Autor(es): |
Oteiza, Juan M.
;
do Prado-Silva, Leonardo
;
Caturla, Magdevis Y. R.
;
Barril, Patricia A.
;
Giannuzzi, Leda
;
Sant'Ana, Anderson S.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FOOD MICROBIOLOGY; v. 124, p. 9-pg., 2024-07-30. |
| Resumo | |
This study aimed to assess the impact of adaptation of ten strains of O157:H7 and non-O157 Escherichia coli to low pH (acid shock or slow acidification) and the effects of this exposure or not on the resistance of E. coli strains to UV radiation in orange juice (pH 3.5). The acid-shocked cells were obtained through culture in tryptic soy broth (TSB) with a final pH of 4.8, which was adjusted by hydrochloric, lactic, or citric acid and subsequently inoculated in orange juice at 4 degrees C for 30 days. No significant differences (p > 0.05) in survival in orange juice were observed between the serotypes O157:H7 and non-O157:H7 for acid-shocked experiments. After slow acidification, where the cells were cultured in TSB supplemented with glucose 1% (TSB + G), a significant increase (p < 0.05) in survival was observed for all strains evaluated. The D-values (radiation dose (J/cm2) necessary to decrease the microbial population by 90%) were determined as the inverse of the slopes of the regressions (k) obtained by plotting log (N/N0). The results show that among the strains tested, E. coli O157:H7 (303/00) and O26:H11 were the most resistant and sensitive strains, respectively. According to our results, the method of acid adaptation contributes to increasing the UV resistance for most of the strains tested. (AU) | |
| Processo FAPESP: | 14/25028-8 - Uso de ferramentas da microbiologia preditiva, metagenômica e metabolômica para estudo da ecologia microbiana da carne bovina embalada a vácuo durante a estocagem |
| Beneficiário: | Magdevis Yanet Rodríguez Caturla |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/20287-9 - Estudo da microbiota deteriorante e estimativa de vida útil de cortes primários de carne bovina por meio de ferramentas da metagenômica e modelagem preditiva |
| Beneficiário: | Magdevis Yanet Rodríguez Caturla |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |