| Texto completo | |
| Autor(es): Mostrar menos - |
Carmona, Rita de Cassia Compagnoli
;
Cilli, Audrey
;
da Costa, Antonio Charlys
;
Reis, Fabricio Caldeira
;
Leal, Elcio
;
dos Santos, Fabiana Cristina Pereira
;
Machado, Braulio Caetano
;
Lopes, Cristina Santiago
;
Afonso, Ana Maria Sardinha
;
Timenetsky, Maria do Carmo Sampaio Tavares
;
Cadar, Daniel
Número total de Autores: 11
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| Tipo de documento: | Artigo Científico |
| Fonte: | MICROORGANISMS; v. 12, n. 1, p. 13-pg., 2024-01-01. |
| Resumo | |
Metagenomic next-generation sequencing (mNGS) methodology serves as an excellent supplement in cases where diagnosis is challenging to establish through conventional laboratory tests, and its usage is increasingly prevalent. Examining the causes of infectious diseases in the central nervous system (CNS) is vital for understanding their spread, managing outbreaks, and effective patient care. In a study conducted in the state of Sao Paulo, Brazil, cerebrospinal fluid (CSF) samples from 500 patients with CNS diseases of indeterminate etiology, collected between 2017 and 2021, were analyzed. Employing a mNGS approach, we obtained the complete coding sequence of Pegivirus hominis (HPgV) genotype 2 in a sample from a patient with encephalitis (named IAL-425/BRA/SP/2019); no other pathogen was detected. Subsequently, to determine the extent of this virus's presence, both polymerase chain reaction (PCR) and/or real-time PCR assays were utilized on the entire collection. The presence of the virus was identified in 4.0% of the samples analyzed. This research constitutes the first report of HPgV detection in CSF samples in South America. Analysis of the IAL-425 genome (9107 nt) revealed a 90% nucleotide identity with HPgV strains from various countries. Evolutionary analyses suggest that HPgV is both endemic and extensively distributed. The direct involvement of HPgV in CNS infections in these patients remains uncertain. (AU) | |
| Processo FAPESP: | 18/21193-5 - EMU concedido no processo 2017/50333-7: sequenciador genético de DNA |
| Beneficiário: | Carlos Henrique Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 17/50333-7 - Plano de desenvolvimento institucional em pesquisa do Instituto Adolfo Lutz (PDIp) |
| Beneficiário: | Carlos Henrique Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa |