| Texto completo | |
| Autor(es): |
Kusebauch, Ulrike
;
Lorenzetti, Alan P. R.
;
Campbell, David S.
;
Pan, Min
;
Shteynberg, David
;
Kapil, Charu
;
Midha, Mukul K.
;
de Lomana, Adrian Lopez Garcia
;
Baliga, Nitin S.
;
Moritz, Robert L.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC DATA; v. 10, n. 1, p. 14-pg., 2023-10-13. |
| Resumo | |
Data-Independent Acquisition (DIA) is a mass spectrometry-based method to reliably identify and reproducibly quantify large fractions of a target proteome. The peptide-centric data analysis strategy employed in DIA requires a priori generated spectral assay libraries. Such assay libraries allow to extract quantitative data in a targeted approach and have been generated for human, mouse, zebrafish, E. coli and few other organisms. However, a spectral assay library for the extreme halophilic archaeon Halobacterium salinarum NRC-1, a model organism that contributed to several notable discoveries, is not publicly available yet. Here, we report a comprehensive spectral assay library to measure 2,563 of 2,646 annotated H. salinarum NRC-1 proteins. We demonstrate the utility of this library by measuring global protein abundances over time under standard growth conditions. The H. salinarum NRC-1 library includes 21,074 distinct peptides representing 97% of the predicted proteome and provides a new, valuable resource to confidently measure and quantify any protein of this archaeon. Data and spectral assay libraries are available via ProteomeXchange (PXD042770, PXD042774) and SWATHAtlas (SAL00312-SAL00319). (AU) | |
| Processo FAPESP: | 17/03052-2 - RNAs não codificantes derivados de sequências de inserção na arqueia halófila Halobacterium salinarum NRC-1 |
| Beneficiário: | Alan Péricles Rodrigues Lorenzetti |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/13440-5 - Detecção de transposases codificadas por Sequências de Inserção usando dados de proteômica |
| Beneficiário: | Alan Péricles Rodrigues Lorenzetti |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |