| Texto completo | |
| Autor(es): Mostrar menos - |
Rocha, Danielly S.
;
Baldo, Thaisa A.
;
Silva-Neto, Habdias A.
;
Duarte-Junior, Gerson F.
;
Bazilio, Gabriela S.
;
Borges, Clayton L.
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Parente-Rocha, Juliana A.
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de Araujo, William R.
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de Siervo, Abner
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Paixa, Thiago L. R. C.
;
Coltro, Wendell K. T.
Número total de Autores: 11
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| Tipo de documento: | Artigo Científico |
| Fonte: | Talanta; v. 268, p. 9-pg., 2023-10-29. |
| Resumo | |
This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protec-tive layer. The COVID-19 diagnostic was based on rapid square wave voltammetry measurements (15 min) using [Fe(CN)6]3-/4-as a redox probe. The method presented a linear response in the concentration range from 250 pg mL-1 to 20 mu g mL-1 S protein, with a limit of detection of 36.3 pg mL-1. The proposed immunosensor was stable for up to two weeks when stored at 4 degrees C and it demonstrated excellent clinical performance in diagnosing COVID-19 when applied to a panel of 44 undiluted swab samples collected from symptomatic patients. In comparison with results obtained through the quantitative reverse transcription polymerase chain reaction method, the proposed immunosensor offered 100 % accuracy, thus emerging as a powerful alternative candidate for routine and decentralized testing, which can be helpful in controlling the COVID-19 outbreak. (AU) | |
| Processo FAPESP: | 07/54829-5 - Estrutura eletrônica e geométrica de nano materiais: um estudo por radiação sincroton |
| Beneficiário: | Richard Landers |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 07/08244-5 - Estudo das propriedades magnéticas de filmes ultrafinos e nanopartículas de Pd e PdAu via XMCD |
| Beneficiário: | Abner de Siervo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/03250-7 - Desenvolvimento de biossensores portáteis e acessíveis para diagnósticos clínicos de infecções bacterianas e virais |
| Beneficiário: | William Reis de Araujo |
| Modalidade de apoio: | Auxílio à Pesquisa - Projeto Inicial |