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Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

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Autor(es):
Calderaro, Franco F. ; Moreno, Luisa Z. ; Doto, Daniela S. ; Matajira, Carlos E. C. ; Gomes, Vasco T. M. ; Ferreira, Thais S. P. ; Mesquita, Renan E. ; Moreno, Andrea M.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Pesquisa Veterinária Brasileira; v. 36, n. 8, p. 4-pg., 2016-08-01.
Resumo

Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp(+)/epf(+)/sly(+) genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed. (AU)

Processo FAPESP: 15/26159-1 - Análise comparativa de genomas de estirpes de Streptococcus suis isoladas de suínos no Brasil
Beneficiário:Carlos Emilio Cabrera Matajira
Modalidade de apoio: Bolsas no Brasil - Doutorado