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da Silva-Junior, Leandro Norberto ; Abreu, Lara de Souza ; Bittencourt Rodrigues, Caroline Fabri ; Galizio, Nathalia da Costa ; Aguiar, Weslei da Silva ; Serino-Silva, Caroline ; dos Santos, Valdomiro Souza ; Costa, Isabella Alves ; Franco Oliveira, Luis Vicente ; Sant'Anna, Savio Stefanini ; Grego, Kathleen Fernandes ; Tanaka-Azevedo, Anita Mitico ; da Silva Rodrigues, Leandro Nascimento ; de Morais-Zani, Karen

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 26, p. 16-pg., 2020-08-26.

Resumo

Background: South American rattlesnakes are represented in Brazil by a single species, Crotalus durissus, which has public health importance due to the severity of its envenomation and to its wide geographical distribution. The species is subdivided into several subspecies, but the current classification is controversial. In Brazil, the venoms of C. d. terrificus and C. d. collilineatus are used for hyperimmunization of horses for antivenom production, even though the distinction of these two subspecies are mostly by their geographical distribution. In this context, we described a comparative compositional and functional characterization of individual C. d. collilineatus and C. d. terrificus venoms from three Brazilian states. Methods: We compared the compositional patterns of C. d. terrificus and C. d. collilineatus individual venoms by 1-DE and RP-HPLC. For functional analyzes, the enzymatic activities of PLA(2), LAAO, and coagulant activity were evaluated. Finally, the immunorecognition of venom toxins by the crotalic antivenom produced at Butantan Institute was evaluated using Western blotting. Results: The protein profile of individual venoms from C. d. collilineatus and C. d. terrificus showed a comparable overall composition, despite some intraspecific variation, especially regarding crotamine and LAAO. Interestingly, HPLC analysis showed a geographic pattern concerning PLA(2). In addition, a remarkable intraspecific variation was also observed in PLA(2), LAAO and coagulant activities. The immunorecognition pattern of individual venoms from C. d. collilineatus and C. d. terrificus by crotalic antivenom produced at Butantan Institute was similar. Conclusions: The results highlighted the individual variability among the venoms of C. durissus ssp. specimens. Importantly, our data point to a geographical variation of C. durissus ssp. venom profile, regardless of the subspecies, as evidenced by PLA(2) isoforms complexity, which may explain the increase in venom neurotoxicity from Northeastern through Southern Brazil reported for the species. (AU)

• Processo FAPESP: 17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/14724-4 - Caracterização proteômica e funcional do veneno de duas espécies de serpentes do gênero Bothrops submetidas à mudança de dieta
• Beneficiário: Nathália da Costa Galizio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/25786-0 - Estudo dos inibidores de fosfolipase A2 (PLIs) presentes nos plasmas das serpentes peçonhentas e não-peçonhentas e avaliação da atividade neutralizante destes sobre as atividades da fosfolipase A2 (PLA2) dos venenos de serpentes
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09976-9 - Comparação do veneno de serpentes Bothrops jararaca em cativeiro e recém-chegadas da natureza
• Beneficiário: Nathália da Costa Galizio
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
• Beneficiário: Karen de Morais Zani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/11108-0 - Estudo comparativo da composição das biomoléculas dos venenos das serpentes Bothrops jararaca e Crotalus durissus terrificus nascidas e mantidas no Instituto Butantan e recém-chegadas da natureza
• Beneficiário: Karen de Morais Zani
• Modalidade de apoio: Auxílio à Pesquisa - Regular