Busca avançada
Ano de início
Entree


Transport of protein disulfide isomerase from the endoplasmic reticulum to the extracellular space without passage through the Golgi complex

Texto completo
Autor(es):
Oliveira, Percillia Victoria Santos ; Dalla Torre, Marco ; Debbas, Victor ; Orsi, Andrea ; Laurindo, Francisco Rafael Martins ; Sitia, Roberto
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Biological Chemistry; v. 300, n. 8, p. 12-pg., 2024-08-01.
Resumo

Protein disulfide isomerase-A1 (PDIA1) is a master regulator of oxidative protein folding and proteostasis in the endoplasmic reticulum (ER). However, PDIA1 can reach the extra cellular space, impacting thrombosis and other pathophysiological phenomena. Whether PDIA1 is externalized via passive release or active secretion is not known. To investigate how PDIA1 negotiates its export, we generated tagged variant that undergoes N-glycosylation in the ER (Glyco-PDIA1). Addition of N-glycans does not alter its enzymatic functions. Upon either deletion of its KDEL ER localization motif or silencing of KDEL receptors, GlycoPDIA1 acquires complex glycans in the Golgi and is secreted. In control cells, however, Glyco-PDIA1 is released with endoglycosidase-H sensitive glycans, implying that it does not follow the classical ER-Golgi route nor does it encounter glycanases in the cytosol. Extracellular Glyco-PDIA1 is more abundant than actin, lactate dehydrogenase, or other proteins released by damaged or dead cells, suggesting active transport through a Golgi-independent route. The strategy we describe herein can be extended to dissect how select ER-residents reach the extracellular space. (AU)

Processo FAPESP: 19/03617-5 - Proteína Dissulfeto Isomerase-A1 (PDIA1) citosólica: um novo mecanismo tiol-redox de sinalização celular
Beneficiário:Percíllia Victória Santos de Oliveira
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 20/03193-8 - Testando a dinâmica de translocação da PDIA1 para o citosol
Beneficiário:Percíllia Victória Santos de Oliveira
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado