| Texto completo | |
| Autor(es): |
Alberice, Juliana V.
;
Funes-Huacca, Maribel E.
;
Carrilho, Emanuel
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of the Brazilian Chemical Society; v. 25, n. 1, p. 7-pg., 2014-01-01. |
| Resumo | |
It was used reverse-transcriptase polymerase chain reaction (RT-PCR) followed by capillary electrophoresis on a microchip to probe the viability of Alicyclobacillus acidoterrestris spores after inactivation with saponin and heat. The method was shown to be suitable for the purpose, and was faster and more sensitive than the traditional plating technique, the standard of the food industry. The limits of quantification and detection were 0.0107 and 0.0039 ng mu L-1 of amplified DNA, respectively. The correlation coefficient (r) between traditional plating and our method was 0.9977, which indicates an excellent correspondence between them. Consequently, it was possible to assess, with confidence, the viability of bacteria using the RT-PCR reaction for detection. It was evaluated the potential of this molecular method over traditional microbiology in the inactivation of A. acidoterrestris by saponin as an effective agent, potentiated by heat. (AU) | |
| Processo FAPESP: | 01/14391-4 - Identificação de Alicyclobacillus acidoterrestris em sucos de laranja pela reação em cadeia da polimerase por transcrição reversa (RT-PCR) e métodos eletroforéticos de análise |
| Beneficiário: | Emanuel Carrilho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |