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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Melatonin reduces LH, 17 beta-estradiol and induces differential regulation of sex steroid receptors in reproductive tissues during rat ovulation

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Autor(es):
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Chuffa, Luiz Gustavo A. [1, 2] ; Seiva, Fabio R. F. [3] ; Favaro, Wagner Jose [1] ; Teixeira, Giovana R. [1] ; Amorim, Joao P. A. [1, 2] ; Mendes, Leonardo O. [1, 2] ; Fioruci, Beatriz A. [1, 2] ; Pinheiro, Patricia Fernanda F. [1] ; Fernandes, Ana Angelica H. [3] ; Franci, Janete A. A. [4] ; Delella, Flavia K. [2] ; Martinez, Marcelo [5] ; Martinez, Francisco E. [1]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Biosci Inst, Dept Anat, BR-18618000 Botucatu, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Struct & Cellular Biol, BR-13083863 Campinas, SP - Brazil
[3] UNESP Univ Estadual Paulista, Biosci Inst, Dept Chem & Biochem, BR-18618000 Botucatu, SP - Brazil
[4] Univ Sao Paulo, Dept Morphol Stomatol & Physiol, BR-14040900 Ribeirao Preto, SP - Brazil
[5] Univ Fed Sao Carlos UFSCar, Dept Morphol & Pathol, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Reproductive Biology and Endocrinology; v. 9, AUG 2 2011.
Citações Web of Science: 38
Resumo

Background: Melatonin is associated with direct or indirect actions upon female reproductive function. However, its effects on sex hormones and steroid receptors during ovulation are not clearly defined. This study aimed to verify whether exposure to long-term melatonin is able to cause reproductive hormonal disturbances as well as their role on sex steroid receptors in the rat ovary, oviduct and uterus during ovulation. Methods: Twenty-four adult Wistar rats, 60 days old (+/-250 g) were randomly divided into two groups. Control group (Co): received 0.9% NaCl 0.3 mL + 95% ethanol 0.04 mL as vehicle; Melatonin-treated group (MEL): received vehicle + melatonin {[} 100 mu g/100 g BW/day] both intraperitoneally during 60 days. All animals were euthanized by decapitation during the morning estrus at 4 a. m. Results: Melatonin significantly reduced the plasma levels of LH and 17 beta-estradiol, while urinary 6-sulfatoximelatonin (STM) was increased at the morning estrus. In addition, melatonin promoted differential regulation of the estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR) and melatonin receptor (MTR) along the reproductive tissues. In ovary, melatonin induced a down-regulation of ER-alpha and PRB levels. Conversely, it was observed that PRA and MT1R were up-regulated. In oviduct, AR and ER-alpha levels were down-regulated, in contrast to high expression of both PRA and PRB. Finally, the ER-beta and PRB levels were down-regulated in uterus tissue and only MT1R was up-regulated. Conclusions: We suggest that melatonin partially suppress the hypothalamus-pituitary-ovarian axis, in addition, it induces differential regulation of sex steroid receptors in the ovary, oviduct and uterus during ovulation. (AU)

Processo FAPESP: 07/59967-7 - Ação da melatonina sobre os receptores estrogênicos ER alfa e ER beta e progestagênicos PRA e PRB nos órgãos genitais femininos internos e o estresse oxidativo nos ovários de ratas adultas UChB (consumidoras voluntárias de etanol a 10%)
Beneficiário:Luiz Gustavo de Almeida Chuffa
Modalidade de apoio: Bolsas no Brasil - Doutorado