Effect of photobiomodulation in an experimental in vitro model of asthma-Copd overlap

Autor(es)

Guerra, Marina Bertoni ; Santana, Kelly Gomes ; Momolli, Marcos ; Labat, Rodrigo ; Chavantes, Maria Cristina ; Zammuner, Stella Regina ; Silva Junior, Jose Antonio ; da Palma, Renata Kelly ; Aimbire, Flavio ; de Oliveira, Ana Paula Ligeiro

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. N/A, p. 9-pg., 2024-08-12.

Resumo

The objective of the study was to evaluate the effect of photobiomodulation (PBM) with laser on the inflammatory process in an experimental in vitro model of ACO. The groups were: (1) human bronchial epithelial cells (BEAS-2B); (2) BEAS-2B cells treated with dexamethasone; (3) BEAS-2B cells irradiated with laser; (4) BEAS-2B cells stimulated with cigarette smoke extract (CSE) + House Dust Mite (HDM); (5) BEAS-2B cells stimulated with CSE + HDM and treated with dexamethasone; (6) BEAS-2B cells incubated with CSE + HDM and irradiated with laser. After 24 h, cytokines were quantified. There was a reduction in TNF-alpha, IL-1 beta, IL-6, IL-4, IL-5, IL-13, IL-17, IL-21, IL-23, and an increase in IL-10 and IFN-gamma in cells from the laser-irradiated ACO group compared to only ACO group. With these results, we can suggest that photobiomodulation acts in the modulation of inflammation observed in ACO, and may be a treatment option. Human bronchial epithelial cells (BEAS-2B) were stimulated with cigarette smoke extract (CSE) and house dust mite (HDM) for induction of the asthma-COPD overlap (ACO). There was an increase in the secretion of inflammatory cytokines in the ACO group. Following photobiomodulation therapy (FBM), a reduction in inflammation was demonstrated with a decrease in the concentration of inflammatory cytokines and increase in IL-10 and IFN-gamma. image (AU)

• Processo FAPESP: 12/16498-5 - Efeito da terapia com laser de baixa potência (LBP) em modelos experimentais de doenças pulmonares crônicas
• Beneficiário: Ana Paula Ligeiro de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores