Fragmentation features of intermolecular cross-linked peptides using N-hydroxy-succinimide esters by MALDI- and ESI-MS/MS for use in structural proteomics

Autor(es)

Santos, Luiz F. A. ^{[1, 2]} ; Iglesias, Amadeu H. ^{[1, 2]} ; Gozzo, Fabio C. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Mass Spectrometry; v. 46, n. 8, p. 742-750, AUG 2011.
• Citações Web of Science: 10

Resumo

The use of mass spectrometry coupled with chemical cross-linking of proteins has become one of the most useful tools for proteins structure and interactions studies. One of the challenges in these studies is the identification of the cross-linked peptides. The interpretation of the MS/MS data generated in cross-linking experiments using N-hydroxy succinimide esters is not trivial once a new amide bond is formed allowing new fragmentation pathways, unlike linear peptides. Intermolecular cross-linked peptides occur when two different peptides are connected by the cross-linker and they yield information on the spatial proximity of different domains (within a protein) or proteins (within a complex). In this article, we report a detailed fragmentation study of intermolecular cross-linked peptides, generated from a set of synthetic peptides, using both ESI and MAID! to generate the precursor ions. The fragmentation features observed here can be helpful in the interpretation and identification of cross-linked peptides present in cross-linking experiments and be further implemented in search engine's algorithms. Copyright (C) 2011 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 07/55930-1 - Desenvolvimento de compostos quinazolínicos inibidores de adenosina quinase para uso terapêutico
• Beneficiário: Kleber Gomes Franchini
• Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Processo FAPESP: 04/14846-0 - Rede de proteoma do estado de São Paulo
• Beneficiário: Fabio Cesar Gozzo
• Linha de fomento: Auxílio à Pesquisa - Programa GENOMA
• Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Linha de fomento: Auxílio à Pesquisa - Temático