Platelet-activating factor receptor (PAFR) regulates neuronal maturation and synaptic transmission during postnatal retinal development

Autor(es)

Dalmaso, Barbara ; Liber, Andre Mauricio Passos ; Ventura, Dora Fix ; Jancar, Sonia ; Del Debbio, Carolina Beltrame

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELLULAR NEUROSCIENCE; v. 18, p. 17-pg., 2024-03-20.

Resumo

Introduction Platelet-activating factor (PAF), PAF receptor (PAFR), and PAF- synthesis/degradation systems are involved in essential CNS processes such as neuroblast proliferation, differentiation, migration, and synaptic modulation. The retina is an important central nervous system (CNS) tissue for visual information processing. During retinal development, the balance between Retinal Progenitor Cell (RPC) proliferation and differentiation is crucial for proper cell determination and retinogenesis. Despite its importance in retinal development, the effects of PAFR deletion on RPC dynamics are still unknown.Methods We compared PAFR knockout mice (PAFR-/-) retinal postnatal development proliferation and differentiation aspects with control animals. Electrophysiological responses were analyzed by electroretinography (ERG).Results and discussion In this study, we demonstrate that PAFR-/- mice increased proliferation during postnatal retinogenesis and altered the expression of specific differentiation markers. The retinas of postnatal PAFR-/- animals decreased neuronal differentiation and synaptic transmission markers, leading to differential responses to light stimuli measured by ERG. Our findings suggest that PAFR signaling plays a critical role in regulating postnatal RPC cell differentiation dynamics during retinal development, cell organization, and neuronal circuitry formation. (AU)

• Processo FAPESP: 20/11352-9 - O papel do receptor do PAF na reprogramação e diferenciação de células-tronco retinianas
• Beneficiário: Bárbara Dalmaso
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/24001-1 - Papel dos microRNAs na regulação das células-tronco retinianas
• Beneficiário: Carolina Beltrame Del Debbio
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/00777-1 - Relação entre hábitos circadianos e funções visuais em serpentes diurnas e noturnas (serpentes, Colubridae)
• Beneficiário: André Maurício Passos Liber
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 22/00191-0 - Mecanismos, genética e desempenho do sistema visual: pesquisa clínica em humanos e experimentação animal
• Beneficiário: Dora Selma Fix Ventura
• Modalidade de apoio: Auxílio à Pesquisa - Temático