| Texto completo | |
| Autor(es): Mostrar menos - |
Camargo, Juliana A.
;
Viana, Nayara I.
;
Pimenta, Ruan
;
Guimaraes, Vanessa R.
;
dos Santos, Gabriel A.
;
Candido, Patricia
;
Ghazarian, Vitoria
;
Romao, Poliana
;
Silva, Iran A.
;
Birbrair, Alexander
;
Srougi, Miguel
;
Nahas, William C.
;
Leite, Katia R.
;
Trarbach, Ericka B.
;
Reis, Sabrina T.
Número total de Autores: 15
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 19, p. 15-pg., 2023-10-01. |
| Resumo | |
Prostate cancer (PCa) has a high prevalence and represents an important health problem, with an increased risk of metastasis. With the advance of CRISPR-Cas9 genome editing, new possibilities have been created for investigating PCa. The technique is effective in knockout oncogenes, reducing tumor resistance. MMP9 and miR-21 target genes are associated with PCa progression; therefore, we evaluated the MMP-9 and miR-21 targets in PCa using the CRISPR-Cas9 system. Single guide RNAs (sgRNAs) of MMP9 and miR-21 sequences were inserted into a PX-330 plasmid, and transfected in DU145 and PC-3 PCa cell lines. MMP9 and RECK expression was assessed by qPCR, WB, and IF. The miR-21 targets, integrins, BAX and mTOR, were evaluated by qPCR. Flow cytometry was performed with Annexin5, 7-AAD and Ki67 markers. Invasion assays were performed with Matrigel. The miR-21 CRISPR-Cas9-edited cells upregulated RECK, MARCKS, BTG2, and PDCD4. CDH1, ITGB3 and ITGB1 were increased in MMP9 and miR-21 CRISPR-Cas9-edited cells. Increased BAX and decreased mTOR were observed in MMP9 and miR-21 CRISPR-Cas9-edited cells. Reduced cell proliferation, increased apoptosis and low invasion in MMP9 and miR-21 edited cells was observed, compared to Scramble. CRISPR-Cas9-edited cells of miR-21 and MMP9 attenuate cell proliferation, invasion and stimulate apoptosis, impeding PCa evolution. (AU) | |
| Processo FAPESP: | 18/19906-3 - Edição genômica com CRISPR/Cas9 para avaliação do papel da MMP9 e seu regulador MIR21 no Câncer de Próstata |
| Beneficiário: | Juliana Alves de Camargo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/19138-9 - Estudo do efeito do miR-137 sobre linhagem celular de câncer de próstata resistente à castração em ambiente hipercolesterolêmico. |
| Beneficiário: | Vitória Ghazarian Nunes |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/17362-3 - Edição genômica com CRISPR/Cas9 para avaliação do papel da MMP9 e de seu regulador MIR21 no câncer de próstata |
| Beneficiário: | Sabrina Thalita dos Reis Faria |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/00156-7 - Estudo in vivo de coativadores e corepressores do receptor de andrógeno no Câncer de Próstata |
| Beneficiário: | Ruan César Aparecido Pimenta |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 22/02288-0 - Avaliação do efeito do silenciamento da MMP9 pelo sistema CRISPR-Cas9 concomitante ao uso do BCG e anti-PDL1 no tratamento do Câncer de Bexiga: estudo in vivo |
| Beneficiário: | Sabrina Thalita dos Reis Faria |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |