| Texto completo | |
| Autor(es): |
Victorio, Jamaira Aparecida
;
Barssotti, Leticia
;
Aprahamian, Tamar
;
Costa, Raul Gobato
;
Mousovich-Neto, Felippe
;
Oliveira, Helena Coutinho Franco
;
Mori, Marcelo
;
Rossoni, Luciana Venturini
;
Davel, Ana Paula
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Endocrinology; v. 165, n. 6, p. 7-pg., 2024-04-29. |
| Resumo | |
Long-term beta-adrenoceptor (beta-AR) stimulation is a pathological mechanism associated with cardiovascular diseases resulting in endothelial and perivascular adipose tissue (PVAT) dysfunction. In this study, we aimed to identify whether beta-adrenergic signaling has a direct effect on PVAT. Thoracic aorta PVAT was obtained from male Wistar rats and cultured ex vivo with the beta-AR agonist isoproterenol (Iso; 1 mu M) or vehicle for 24 hours. Conditioned culture medium (CCM) from Iso-treated PVAT induced a marked increase in aorta contractile response, induced oxidative stress, and reduced nitric oxide production in PVAT compared to vehicle. In addition, Iso-treated PVAT and PVAT-derived differentiated adipocytes exhibited higher corticosterone release and protein expression of 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1), an enzyme responsible for de novo synthesis of corticosterone. Macrophages exposed to Iso also exhibited increased corticosterone release in response to beta-AR stimulation. Incubation of Iso-treated PVAT and PVAT-derived differentiated adipocytes with beta 3-AR antagonist restored aorta contractile function modulated by Iso-CCM and normalized 11 beta-HSD1 protein expression. These results show that beta 3-AR signaling leads to upregulation of 11 beta-HSD1 in PVAT, thus increasing corticosterone release and contributing to impair the anticontractile function of this tissue. (AU) | |
| Processo FAPESP: | 14/07947-6 - Possível interação entre estimulação beta-adrenérgica e ativação do receptor de mineralocorticóide em células endoteliais, musculares lisas e do tecido adiposo perivascular |
| Beneficiário: | Ana Paula Couto Davel |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/16505-8 - Disfunção e inflamação do tecido adiposo perivascular na obesidade: papel do receptor de mineralocorticoides |
| Beneficiário: | Ana Paula Couto Davel |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |