| Texto completo | |
| Autor(es): |
Alvarengaa, Elenice M.
[1]
;
Mondin, Mateus
[2]
;
Martins, James A.
[1]
;
Rodrigues, Vera L. C. C.
[3]
;
Vidal, Benedicto C.
[1]
;
Rincones, Johana
[4]
;
Carazzolle, Marcelo F.
[4]
;
Andrade, Larissa Mara
[2]
;
Mello, Maria Luiza S.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Biol, Dept Anat Cell Biol & Physiol, BR-13083863 Campinas, SP - Brazil
[2] Univ Sao Paulo, Dept Genet, Luiz de Queiroz Coll Agr ESALQ, BR-13418900 Piracicaba, SP - Brazil
[3] SUCEN, BR-13840000 Mogi Guacu, SP - Brazil
[4] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo de Revisão |
| Fonte: | Micron; v. 42, n. 6, p. 568-578, AUG 2011. |
| Citações Web of Science: | 8 |
| Resumo | |
Heterochromatin bodies in single- and multichromocentered interphase cell nuclei of Triatoma infestans, a vector of Chagas disease, have been suggested to contain AT-rich DNA, based on their positive response to Q-banding and Hoechst 33248 treatment. No information exists on whether GC-rich DNA is also present in these nuclei and whether it plays a role on chromatin condensation. Considering that methodologies more precise than those previously used to determine DNA base composition in situ are currently available, and that the spatial distribution of chromatin areas differing in composition in interphase cell nuclei of different species is a matter of interest, the localization of AT- and GC-rich DNA in T. infestans nuclei is revisited here. The methodologies used included DAPI/AMD and CMA(3)/Distamycin differential staining, Feulgen-DNA image analysis following Msp I and Hpa II enzymatic digestion, 5-methylcytidine immunodetection, AgNOR response, confocal microscopy, and the 5-aza-2'-deoxycytidine (5-AZA) demethylation assay. The results identified the presence of AT-rich/GC-poor DNA in chromocenters and evenly distributed AT and GC sequences in euchromatin. A GC-rich DNA zone encircling the chromocenters was also found but it could not be associated with NOR regions. To corroborate the DNA AT-richness in T. infestans nuclei, bioinformatic analyses were also performed. Methylated cytosine was evident at some points of the chromocenters' edge in single- and multichromocentered nuclei and at the euchromatin of multichromocentered nuclei and could be transiently affected by the 5-AZA treatment. The present results suggest that in the particular case of chromocenters of the hemipteran T. infestans, cytosine methylation is not a relevant factor involved in chromatin condensation. (C) 2011 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 10/50015-6 - Estrutura e organização da cromatina com o envelhecimento e o diabetes frente a alterações induzidas em marcadores epigenéticos |
| Beneficiário: | Maria Luiza Silveira Mello |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |