Biogenic silver nanoparticles' antibacterial activity and cytotoxicity on human hepatocarcinoma cells (Huh-7)

Autor(es)

da Costa, Thyerre Santana ; da Silva, Mariana Rodrigues ; Barbosa, Julio Cesar Jeronimo ; Das Neves, Uedson Da Silva ; de Jesus, Marcelo Bispo ; Tasic, Ljubica

• Tipo de documento: Artigo Científico
• Fonte: RSC ADVANCES; v. 14, n. 4, p. 13-pg., 2024-01-10.

Resumo

Exploring diverse synthetic pathways for nanomaterial synthesis has emerged as a promising direction. For example, silver nanoparticles (AgNPs) are synthesized using different approaches yielding nanomaterials with distinct morphological, physical and biological properties. Hence, the present study reports the biogenic synthesis of silver nanoparticles using the aqueous secretome of the fungus Fusarium oxysporum f. sp. cubense (AgNP@Fo) and orange peel extract (AgNP@OR). The physical and morphological properties of synthesized nanoparticles were similar, with AgNP@Fo measuring 56.43 +/- 19.18 nm and AgNP@OR measuring 39.97 +/- 19.72 nm in size. The zeta potentials for the nanoparticles were low, -26.8 +/- 7.55 and -26.2 +/- 2.87 mV for AgNP@Fo and AgNP@OR, respectively, demonstrating a similar negative charge. The spherical morphologies of both nanoparticles were evidenced by Scanning Transmission Electron Microscopy (STEM) and Atomic Force Microscopy (AFM). However, despite their similar physical and morphological properties, AgNPs demonstrated different bioactivities. We evaluated and compared the antimicrobial efficacy of these nanoparticles against a range of bacteria, such as Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. The AgNP@Fo showed Minimum Inhibitory Concentration (MIC) values ranging from 0.84 to 1.68 mu g mL-1 and were around ten times more potent compared to AgNP@OR. The anticancer activities of both nanoparticles were investigated using human hepatocarcinoma cells (Huh-7), where AgNP@Fo exhibited around 20 times higher cytotoxicity than AgNP@OR with an IC50 value of 0.545 mu mol L-1. Anticancer effects were demonstrated by the MTT, confirmed by the calcein-AM assay and fluorescence imaging. This study establishes solid groundwork for future exploration of molecular interactions of nanoparticles synthesized through distinct biosynthetic routes, particularly within bacterial and cancerous cell environments. This study aimed to explore the antibacterial and anticancer potential of biosynthesized silver nanoparticles and how the biosynthesis of AgNP@Bio determines their biological roles. (AU)

• Processo FAPESP: 23/02338-0 - Anotação e identificação de metabólitos em células selvagens e modificadas usando abordagem de RMN não direcionada e integrada
• Beneficiário: Thyerre Santana da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 23/06874-4 - Decifrando o papel de metabólitos secundários na interação química entre fitopatógenos e o hospedeiro citros
• Beneficiário: Júlio César Jerônimo Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/07854-4 - Determinação de alterações fenotípicas subletais desencadeadas por microplásticos em dois modelos de hepatócitos humanos através de triagem celular multiparamétrica de alto conteúdo
• Beneficiário: Mariana Rodrigues da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 20/08615-8 - Exosítios de proteínas, sítios crípticos e moonlighting: identificação, mapeamento funcional e efeitos de alteração estrutural
• Beneficiário: Raghuvir Krishnaswamy Arni
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/01218-3 - Nanotoxicidade analisada individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Regular